Rearrangement and mutagenesis of a shuttle vector plasmid after passage in mammalian cells - PubMed (original) (raw)
Rearrangement and mutagenesis of a shuttle vector plasmid after passage in mammalian cells
A Razzaque et al. Proc Natl Acad Sci U S A. 1983 May.
Abstract
A shuttle vector plasmid that contains sequences from simian virus 40, pBR322, and a bacterial marker gene, galactokinase, has been constructed. After replication in cells permissive for virus progeny, plasmid DNA was introduced into a galactokinase-deficient bacterial strain and the relative frequency of colonies with plasmids but without galactokinase activity was determined. This assay showed that 1% of the plasmids were defective after passage in the mammalian cells. Individual mutant plasmids were examined and found to contain deletions, duplications, point mutations, and insertions of cell DNA.
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