A nuclear mutation prevents processing of a mitochondrially encoded membrane protein in Saccharomyces cerevisiae - PubMed (original) (raw)

Comparative Study

A nuclear mutation prevents processing of a mitochondrially encoded membrane protein in Saccharomyces cerevisiae

E Pratje et al. EMBO J. 1983.

Abstract

Subunit II of cytochrome oxidase is encoded by the mitochondrial OXI1 gene in Saccharomyces cerevisiae. The temperature-sensitive nuclear pet mutant ts2858 has an apparent higher mol. wt. subunit II when analyzed on lithium dodecylsulfate (LiDS) polyacrylamide gels. However, on LiDS-6M urea gels the apparent mol. wt. of the wild-type protein exceeds that of the mutant. Partial revertants of mutant ts2858 that produce both the wild-type and mutant form of subunit II were isolated. The two forms of subunit II differ at the N-terminal part of the molecule as shown by constructing and analyzing nuclear ts2858 and mitochondrial chain termination double mutants. The presence of the primary translation product in the mutant and of the processed form in the wild-type lacking 15 amino-terminal residues was demonstrated by radiolabel protein sequencing. Comparison of the known DNA sequence with the partial protein sequence obtained reveals that six of the 15 residues are hydrophilic and, unlike most signal sequences, this transient sequence does not contain extended hydrophobic parts. The nuclear mutation ts2858 preventing post-translational processing of cytochrome oxidase subunit II lies either in the gene for a protease or an enzyme regulating a protease.

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References

    1. Proc Natl Acad Sci U S A. 1972 Apr;69(4):930-2 - PubMed
    1. J Biol Chem. 1975 Jan 25;250(2):752-61 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1083-6 - PubMed
    1. J Biol Chem. 1977 Feb 10;252(3):1102-6 - PubMed
    1. J Biol Chem. 1978 Jan 10;253(1):297-304 - PubMed

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