Insulin stimulates phosphorylation of serine residues in soluble insulin receptors - PubMed (original) (raw)

Insulin stimulates phosphorylation of serine residues in soluble insulin receptors

Y Zick et al. Biochem Biophys Res Commun. 1983.

Abstract

Using lectin affinity-purified receptor preparations from human hepatoma cells, insulin (10(-7)M) specifically stimulated phosphorylation of the 95,000 dalton (beta) subunit of its own receptor. Phospho-amino acid analysis of the receptor subunit revealed that insulin increased at least 2.5-fold the content of phosphoserine and of phosphotyrosine. In intact cells, the major effect of insulin is to increase the phosphoserine content of its receptor. These findings are the first demonstration of an insulin-stimulated serine kinase in a cell-free system.

PubMed Disclaimer

Similar articles

• Insulin stimulation of phosphorylation of the beta subunit of the insulin receptor. Formation of both phosphoserine and phosphotyrosine.
  Kasuga M, Zick Y, Blith DL, Karlsson FA, Häring HU, Kahn CR. Kasuga M, et al. J Biol Chem. 1982 Sep 10;257(17):9891-4. J Biol Chem. 1982. PMID: 6179940
• Phorbol esters modulate insulin receptor phosphorylation and insulin action in cultured hepatoma cells.
  Takayama S, White MF, Lauris V, Kahn CR. Takayama S, et al. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7797-801. doi: 10.1073/pnas.81.24.7797. Proc Natl Acad Sci U S A. 1984. PMID: 6393128 Free PMC article.
• The nature and regulation of the insulin receptor: structure and function.
  Czech MP. Czech MP. Annu Rev Physiol. 1985;47:357-81. doi: 10.1146/annurev.ph.47.030185.002041. Annu Rev Physiol. 1985. PMID: 2986534 Review.

Cited by

• Insulin and insulin-like growth factor receptors in the nervous system.
  Adamo M, Raizada MK, LeRoith D. Adamo M, et al. Mol Neurobiol. 1989 Spring-Summer;3(1-2):71-100. doi: 10.1007/BF02935589. Mol Neurobiol. 1989. PMID: 2553069 Review.
• Catalysis of serine and tyrosine autophosphorylation by the human insulin receptor.
  Baltensperger K, Lewis RE, Woon CW, Vissavajjhala P, Ross AH, Czech MP. Baltensperger K, et al. Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):7885-9. doi: 10.1073/pnas.89.17.7885. Proc Natl Acad Sci U S A. 1992. PMID: 1381504 Free PMC article.
• Hyperinsulinemia and Its Pivotal Role in Aging, Obesity, Type 2 Diabetes, Cardiovascular Disease and Cancer.
  Janssen JAMJL. Janssen JAMJL. Int J Mol Sci. 2021 Jul 21;22(15):7797. doi: 10.3390/ijms22157797. Int J Mol Sci. 2021. PMID: 34360563 Free PMC article. Review.
• Two systems in vitro that show insulin-stimulated serine kinase activity towards the insulin receptor.
  Smith DM, King MJ, Sale GJ. Smith DM, et al. Biochem J. 1988 Mar 1;250(2):509-19. doi: 10.1042/bj2500509. Biochem J. 1988. PMID: 2965579 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Medical

  • MedlinePlus Health Information