DNA-binding properties of simian virus 40 T-antigen mutants defective in viral DNA replication - PubMed (original) (raw)
Comparative Study
DNA-binding properties of simian virus 40 T-antigen mutants defective in viral DNA replication
C Prives et al. Mol Cell Biol. 1983 Nov.
Abstract
Three simian virus 40 (SV40)-transformed monkey cell lines, C2, C6, and C11, producing T-antigen variants that are unable to initiate viral DNA replication, were analyzed with respect to their affinity for regulatory sequences at the viral origin of replication. C2 and C11 T antigens both bound specifically to sequences at sites 1 and 2 at the viral origin region, whereas C6 T antigen showed no specific affinity for any viral DNA sequences under all conditions tested. Viral DNA sequences encoding the C6 T antigen have recently been cloned out of C6 cells and used to transform an established rat cell line. T antigen from several cloned C6-SV40-transformed rat lines failed to bind specifically to the origin. C6 DNA contains three mutations: two located close to the amino terminus of T antigen at amino acid positions 30 and 51 and a third located internally at amino acid position 153. Two recombinant SV40 DNA mutants were prepared containing either the amino-terminal mutations at positions 30 and 51 (C6-1) or the internally located mutation at position 153 (C6-2) and used to transform Rat 2 cells. Whereas T antigen from C6-2-transformed cells lacked any specific affinity for these sequences. Therefore, the single mutation at amino acid position 153 (Asn leads to Thr) is sufficient to abolish the origin-binding property of T antigen. A T antigen-specific monoclonal antibody, PAb 100, which had been previously shown to immunoprecipitate an immunologically distinct origin-binding subclass of T antigen, recognized wild-type or C6-1 antigens, but failed to react with C6 or C6-2 T antigens. These results indicate that viral replication function comprises properties of T antigen that exist in addition to its ability to bind specifically to the SV40 regulatory sequences. Furthermore, it is concluded from these data that specific viral origin binding is not a necessary feature of the transforming function of T antigen.
Similar articles
- Simian virus 40 large T-antigen point mutants that are defective in viral DNA replication but competent in oncogenic transformation.
Manos MM, Gluzman Y. Manos MM, et al. Mol Cell Biol. 1984 Jun;4(6):1125-33. doi: 10.1128/mcb.4.6.1125-1133.1984. Mol Cell Biol. 1984. PMID: 6330530 Free PMC article. - Association of simian virus 40 T antigen with the nuclear matrix of infected and transformed monkey cells.
Covey L, Choi Y, Prives C. Covey L, et al. Mol Cell Biol. 1984 Jul;4(7):1384-92. doi: 10.1128/mcb.4.7.1384-1392.1984. Mol Cell Biol. 1984. PMID: 6095067 Free PMC article. - Genetic and biochemical analysis of transformation-competent, replication-defective simian virus 40 large T antigen mutants.
Manos MM, Gluzman Y. Manos MM, et al. J Virol. 1985 Jan;53(1):120-7. doi: 10.1128/JVI.53.1.120-127.1985. J Virol. 1985. PMID: 2981330 Free PMC article. - Properties of the simian virus 40 (SV40) large T antigens encoded by SV40 mutants with deletions in gene A.
Cole CN, Tornow J, Clark R, Tjian R. Cole CN, et al. J Virol. 1986 Feb;57(2):539-46. doi: 10.1128/JVI.57.2.539-546.1986. J Virol. 1986. PMID: 3003386 Free PMC article. - A small subclass of SV40 T antigen binds to the viral origin of replication.
Scheller A, Covey L, Barnet B, Prives C. Scheller A, et al. Cell. 1982 Jun;29(2):375-83. doi: 10.1016/0092-8674(82)90154-4. Cell. 1982. PMID: 6288256
Cited by
- Quantitative analysis of viral load per haploid genome revealed the different biological features of Merkel cell polyomavirus infection in skin tumor.
Ota S, Ishikawa S, Takazawa Y, Goto A, Fujii T, Ohashi K, Fukayama M. Ota S, et al. PLoS One. 2012;7(6):e39954. doi: 10.1371/journal.pone.0039954. Epub 2012 Jun 29. PLoS One. 2012. PMID: 22768181 Free PMC article. - Wild-type p53 enhances efficiency of simian virus 40 large-T-antigen-induced cellular transformation.
Hermannstädter A, Ziegler C, Kühl M, Deppert W, Tolstonog GV. Hermannstädter A, et al. J Virol. 2009 Oct;83(19):10106-18. doi: 10.1128/JVI.00174-09. Epub 2009 Jul 22. J Virol. 2009. PMID: 19625393 Free PMC article. - T antigen mutations are a human tumor-specific signature for Merkel cell polyomavirus.
Shuda M, Feng H, Kwun HJ, Rosen ST, Gjoerup O, Moore PS, Chang Y. Shuda M, et al. Proc Natl Acad Sci U S A. 2008 Oct 21;105(42):16272-7. doi: 10.1073/pnas.0806526105. Epub 2008 Sep 23. Proc Natl Acad Sci U S A. 2008. PMID: 18812503 Free PMC article. - Analyses of the interaction between the origin binding domain from simian virus 40 T antigen and single-stranded DNA provide insights into DNA unwinding and initiation of DNA replication.
Reese DK, Meinke G, Kumar A, Moine S, Chen K, Sudmeier JL, Bachovchin W, Bohm A, Bullock PA. Reese DK, et al. J Virol. 2006 Dec;80(24):12248-59. doi: 10.1128/JVI.01201-06. Epub 2006 Sep 27. J Virol. 2006. PMID: 17005644 Free PMC article. - Simian virus 40 large T-antigen point mutants that are defective in viral DNA replication but competent in oncogenic transformation.
Manos MM, Gluzman Y. Manos MM, et al. Mol Cell Biol. 1984 Jun;4(6):1125-33. doi: 10.1128/mcb.4.6.1125-1133.1984. Mol Cell Biol. 1984. PMID: 6330530 Free PMC article.
References
- J Mol Biol. 1967 Jun 14;26(2):365-9 - PubMed
- J Virol. 1977 Feb;21(2):647-57 - PubMed
- J Virol. 1977 May;22(2):256-66 - PubMed
- J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
- Cell. 1978 Jan;13(1):165-79 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous