Novel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloning - PubMed (original) (raw)
Novel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloning
F L Macrina et al. Gene. 1983 Nov.
Free article
Abstract
A novel plasmid vector that is able to replicate both in Escherichia coli and in Streptococcus sanguis is described. This 9.2-kb plasmid, designated pVA856, carries Cmr, Tcr, and Emr determinants that are expressed in E. coli. Only the Emr determinant is expressed in S. sanguis. Both the Cmr and the Tcr of pVA856 may be insertionally inactivated. This plasmid affords several different cleavage-ligation strategies for cloning in E. coli followed by subsequent introduction of chimeras into S. sanguis. In addition, we have modified a previously described E. coli-S. sanguis shuttle plasmid [pVA838; Macrina et al., Gene 19 (1982) 345-353], so that it is unable to replicate in S. sanguis. The utility of such a plasmid for cloning and selecting sequences enabling autonomous replication in S. sanguis is demonstrated.
Similar articles
- A cloning vector able to replicate in Escherichia coli and Streptococcus sanguis.
Macrina FL, Tobian JA, Jones KR, Evans RP, Clewell DB. Macrina FL, et al. Gene. 1982 Oct;19(3):345-53. doi: 10.1016/0378-1119(82)90025-7. Gene. 1982. PMID: 6295886 - Return of Streptococcus faecalis DNA cloned in Escherichia coli to its original host via transformation of Streptococcus sanguis followed by conjugative mobilization.
Smith MD, Clewell DB. Smith MD, et al. J Bacteriol. 1984 Dec;160(3):1109-14. doi: 10.1128/jb.160.3.1109-1114.1984. J Bacteriol. 1984. PMID: 6094500 Free PMC article. - Novel streptococcal-integration shuttle vectors for gene cloning and inactivation.
Tao L, LeBlanc DJ, Ferretti JJ. Tao L, et al. Gene. 1992 Oct 12;120(1):105-10. doi: 10.1016/0378-1119(92)90016-i. Gene. 1992. PMID: 1327968 - Construction and characterization of shuttle plasmids for lactic acid bacteria and Escherichia coli.
Solaiman DK, Somkuti GA, Steinberg DH. Solaiman DK, et al. Plasmid. 1992 Jul;28(1):25-36. doi: 10.1016/0147-619x(92)90033-7. Plasmid. 1992. PMID: 1518910 - Cloning and characterization of DNAs with palindromic sequences.
Leach DR. Leach DR. Genet Eng (N Y). 1996;18:1-11. doi: 10.1007/978-1-4899-1766-9_1. Genet Eng (N Y). 1996. PMID: 8785115 Review. No abstract available.
Cited by
- The Streptococcus gordonii platelet binding protein GspB undergoes glycosylation independently of export.
Bensing BA, Gibson BW, Sullam PM. Bensing BA, et al. J Bacteriol. 2004 Feb;186(3):638-45. doi: 10.1128/JB.186.3.638-645.2004. J Bacteriol. 2004. PMID: 14729688 Free PMC article. - The Streptococcus gordonii surface proteins GspB and Hsa mediate binding to sialylated carbohydrate epitopes on the platelet membrane glycoprotein Ibalpha.
Bensing BA, López JA, Sullam PM. Bensing BA, et al. Infect Immun. 2004 Nov;72(11):6528-37. doi: 10.1128/IAI.72.11.6528-6537.2004. Infect Immun. 2004. PMID: 15501784 Free PMC article. - Study of Staphylococcus aureus pathogenic genes by transfer and expression in the less virulent organism Streptococcus gordonii.
Stutzmann Meier P, Entenza JM, Vaudaux P, Francioli P, Glauser MP, Moreillon P. Stutzmann Meier P, et al. Infect Immun. 2001 Feb;69(2):657-64. doi: 10.1128/IAI.69.2.657-664.2001. Infect Immun. 2001. PMID: 11159952 Free PMC article. - Characterization of a P1-deficient strain of Streptococcus mutans that expresses the SpaA protein of Streptococcus sobrinus.
Kuykindoll RJ, Holt RG. Kuykindoll RJ, et al. Infect Immun. 1996 Sep;64(9):3652-8. doi: 10.1128/iai.64.9.3652-3658.1996. Infect Immun. 1996. PMID: 8751913 Free PMC article. - Cloning and sequence analysis of the gbpC gene encoding a novel glucan-binding protein of Streptococcus mutans.
Sato Y, Yamamoto Y, Kizaki H. Sato Y, et al. Infect Immun. 1997 Feb;65(2):668-75. doi: 10.1128/iai.65.2.668-675.1997. Infect Immun. 1997. PMID: 9009329 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources