Two improved promoter sequences for the beta-lactamase expression arising from a single base-pair substitution - PubMed (original) (raw)

Two improved promoter sequences for the beta-lactamase expression arising from a single base-pair substitution

S T Chen et al. Nucleic Acids Res. 1984.

Free PMC article

Abstract

A mutation in the transposon Tn2660 derived from the plasmid R6K, and resulting in an approximate tenfold increase in penicillin resistance is shown to be a single GC to AT substitution 177 base pairs 'upstream' of the initiation codon of the structural beta-lactamase (bla) gene. This substitution leads to the transcription of two new mRNAs which can be ascribed to the creation of two new overlapping promoter sequences. All the sequences (450bp) examined in the wild-type Tn2660 are identical to those reported in Tn3.

PubMed Disclaimer

Similar articles

• Characterization of the beta-lactamase promoter of pBR322.
  Russell DR, Bennett GN. Russell DR, et al. Nucleic Acids Res. 1981 Jun 11;9(11):2517-33. doi: 10.1093/nar/9.11.2517. Nucleic Acids Res. 1981. PMID: 6269053 Free PMC article.
• Variations between the nucleotide sequences of Tn1, Tn2, and Tn3 and expression of beta-lactamase in Pseudomonas aeruginosa and Escherichia coli.
  Chen ST, Clowes RC. Chen ST, et al. J Bacteriol. 1987 Feb;169(2):913-6. doi: 10.1128/jb.169.2.913-916.1987. J Bacteriol. 1987. PMID: 3027053 Free PMC article.
• Far upstream sequences of the bla promoter from TN3 are involved in complexation with E. coli RNA-polymerase.
  Duval-Valentin G, Ehrlich R. Duval-Valentin G, et al. Nucleic Acids Res. 1988 Mar 25;16(5):2031-44. doi: 10.1093/nar/16.5.2031. Nucleic Acids Res. 1988. PMID: 2833726 Free PMC article.
• Primary structure of an aminoglycoside 6'-N-acetyltransferase AAC(6')-4, fused in vivo with the signal peptide of the Tn3-encoded beta-lactamase.
  Tran van Nhieu G, Collatz E. Tran van Nhieu G, et al. J Bacteriol. 1987 Dec;169(12):5708-14. doi: 10.1128/jb.169.12.5708-5714.1987. J Bacteriol. 1987. PMID: 2824444 Free PMC article.

Cited by

• Transferable hyperproduction of TEM-1 beta-lactamase in Shigella flexneri due to a point mutation in the pribnow box.
  Siu LK, Ho PL, Yuen KY, Wong SS, Chau PY. Siu LK, et al. Antimicrob Agents Chemother. 1997 Feb;41(2):468-70. doi: 10.1128/AAC.41.2.468. Antimicrob Agents Chemother. 1997. PMID: 9021210 Free PMC article.
• Ambler class A extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella spp. in Canadian hospitals.
  Mulvey MR, Bryce E, Boyd D, Ofner-Agostini M, Christianson S, Simor AE, Paton S; Canadian Hospital Epidemiology Committee, Canadian Nosocomial Infection Surveillance Program, Health Canada. Mulvey MR, et al. Antimicrob Agents Chemother. 2004 Apr;48(4):1204-14. doi: 10.1128/AAC.48.4.1204-1214.2004. Antimicrob Agents Chemother. 2004. PMID: 15047521 Free PMC article.
• Mechanisms of decreased susceptibility to cefpodoxime in Escherichia coli.
  Oliver A, Weigel LM, Rasheed JK, McGowan JE Jr, Raney P, Tenover FC. Oliver A, et al. Antimicrob Agents Chemother. 2002 Dec;46(12):3829-36. doi: 10.1128/AAC.46.12.3829-3836.2002. Antimicrob Agents Chemother. 2002. PMID: 12435684 Free PMC article.
• Updated sequence information for TEM beta-lactamase genes.
  Goussard S, Courvalin P. Goussard S, et al. Antimicrob Agents Chemother. 1999 Feb;43(2):367-70. doi: 10.1128/AAC.43.2.367. Antimicrob Agents Chemother. 1999. PMID: 9925535 Free PMC article.
• Promoters P3, Pa/Pb, P4, and P5 upstream from bla(TEM) genes and their relationship to beta-lactam resistance.
  Lartigue MF, Leflon-Guibout V, Poirel L, Nordmann P, Nicolas-Chanoine MH. Lartigue MF, et al. Antimicrob Agents Chemother. 2002 Dec;46(12):4035-7. doi: 10.1128/AAC.46.12.4035-4037.2002. Antimicrob Agents Chemother. 2002. PMID: 12435720 Free PMC article.

References

1. 1. Nature. 1965 Oct 16;208(5007):239-41 - PubMed
2. 1. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3232-6 - PubMed
3. 1. J Bacteriol. 1973 Jun;114(3):1116-24 - PubMed
4. 1. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455-9 - PubMed
5. 1. J Bacteriol. 1975 Apr;122(1):250-6 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations Database

• Miscellaneous

  • NCI CPTAC Assay Portal