Analysis of DNA sequences which regulate the transcription of a herpes simplex virus immediate early gene - PubMed (original) (raw)
Analysis of DNA sequences which regulate the transcription of a herpes simplex virus immediate early gene
C M Preston et al. J Virol. 1984 Jun.
Abstract
The locations and functions of DNA sequences involved in transcription of the gene encoding herpes simplex virus type 1 immediate early (IE) mRNAs 4 and 5 were analyzed by use of a transient-expression assay. The region upstream of the genes encoding IE mRNAs 4 and 5 was fused to the thymidine kinase gene coding sequences, and production of enzyme or RNA was measured after transfection of plasmids into BHK cells. The effect of deletions in the upstream region was determined in the absence or presence of a virus structural component which stimulates herpes simplex virus IE transcription. Two distinct units were identified. One of these was a promoter which required not more than 69 base pairs of DNA specific for the genes encoding IE mRNAs 4 and 5 upstream from the mRNA 5' terminus. The other was a far-upstream region which mediated the response to the virion component and had an upstream boundary between nucleotides -347 and -335. An origin of DNA replication was interposed between these two units. The element TAATGAGATAC , which represents a consensus sequence present in the upstream regions of all herpes simplex type 1 IE genes, appeared to be essential for stimulation by the virion component. The activity of this element was modulated by the sequences which flank it, especially by regions having extremely high contents of guanine plus cytosine and which contain a conserved unit CCCGCCC or its complement GGGCGGG .
Similar articles
- Identification of herpes simplex virus DNA sequences which encode a trans-acting polypeptide responsible for stimulation of immediate early transcription.
Campbell ME, Palfreyman JW, Preston CM. Campbell ME, et al. J Mol Biol. 1984 Nov 25;180(1):1-19. doi: 10.1016/0022-2836(84)90427-3. J Mol Biol. 1984. PMID: 6096556 - DNA sequences which regulate the expression of the pseudorabies virus major immediate early gene.
Campbell ME, Preston CM. Campbell ME, et al. Virology. 1987 Apr;157(2):307-16. doi: 10.1016/0042-6822(87)90273-x. Virology. 1987. PMID: 3029974 - Identification of the herpes simplex virus type 1 gene encoding the dUTPase.
Preston VG, Fisher FB. Preston VG, et al. Virology. 1984 Oct 15;138(1):58-68. doi: 10.1016/0042-6822(84)90147-8. Virology. 1984. PMID: 6149651 - Functional analysis of a herpes simplex virus type 1 promoter: identification of far-upstream regulatory sequences.
Cordingley MG, Campbell ME, Preston CM. Cordingley MG, et al. Nucleic Acids Res. 1983 Apr 25;11(8):2347-65. doi: 10.1093/nar/11.8.2347. Nucleic Acids Res. 1983. PMID: 6304649 Free PMC article.
Cited by
- Sequence and function of canine herpesvirus alpha-transinducing factor and its interaction with an immediate early promoter.
Tyack SG, Studdert MJ, Johnson MA. Tyack SG, et al. Virus Genes. 2006 Dec;33(3):299-307. doi: 10.1007/s11262-006-0069-5. Virus Genes. 2006. PMID: 16991001 - The Epstein-Barr virus lytic program is controlled by the co-operative functions of two transactivators.
Feederle R, Kost M, Baumann M, Janz A, Drouet E, Hammerschmidt W, Delecluse HJ. Feederle R, et al. EMBO J. 2000 Jun 15;19(12):3080-9. doi: 10.1093/emboj/19.12.3080. EMBO J. 2000. PMID: 10856251 Free PMC article. - Potential role for luman, the cellular homologue of herpes simplex virus VP16 (alpha gene trans-inducing factor), in herpesvirus latency.
Lu R, Misra V. Lu R, et al. J Virol. 2000 Jan;74(2):934-43. doi: 10.1128/jvi.74.2.934-943.2000. J Virol. 2000. PMID: 10623756 Free PMC article. - Cellular transcription factors enhance herpes simplex virus type 1 oriS-dependent DNA replication.
Nguyen-Huynh AT, Schaffer PA. Nguyen-Huynh AT, et al. J Virol. 1998 May;72(5):3635-45. doi: 10.1128/JVI.72.5.3635-3645.1998. J Virol. 1998. PMID: 9557644 Free PMC article. - Concerted activity of host cell factor subregions in promoting stable VP16 complex assembly and preventing interference by the acidic activation domain.
LaBoissière S, Walker S, O'Hare P. LaBoissière S, et al. Mol Cell Biol. 1997 Dec;17(12):7108-18. doi: 10.1128/MCB.17.12.7108. Mol Cell Biol. 1997. PMID: 9372942 Free PMC article.
References
- Virology. 1975 Jul;66(1):140-50 - PubMed
- Science. 1983 Feb 11;219(4585):626-31 - PubMed
- J Gen Virol. 1976 Dec;33(3):447-58 - PubMed
- Virology. 1977 May 1;78(1):349-53 - PubMed
- Cell. 1977 Sep;12(1):275-85 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources