In vitro interaction of Mycobacterium avium with intestinal epithelial cells - PubMed (original) (raw)
In vitro interaction of Mycobacterium avium with intestinal epithelial cells
M E Mapother et al. Infect Immun. 1984 Jul.
Abstract
Human intestinal epithelial cell monolayers were inoculated with cultures of Mycobacterium avium serotype 2, 8, or 10 that were viable, autoclaved, Formalin killed, exposed to UV light, or suspended in anti-M. avium serotype 2 serum. The effects of four reagents known to block phagocytosis or endocytosis (cytochalasin B, dibutyryl cyclic adenosine monophosphate, iodoacetate, and 2,4-dinitrophenol) on the bacteria-cell interaction were also studied. The maximum uptake of pathogenic M. avium by human intestinal epithelial cells occurred after 2 to 3 h of incubation. Serotype 2 was taken up in greater quantity than serotype 8 or 10. Saprophytic mycobacteria did not attach to or penetrate the host cells. The data showed that viable mycobacteria are ingested by host cells, whereas dead organisms are not. Components of the bacterial cells are partially, but not solely, responsible for the phagocytosis of M. avium serotype 2 by human intestinal epithelial cells. Furthermore, uptake of M. avium by human intestinal epithelial cells was suppressed by reagents which inhibit uptake by known phagocytic cells, suggesting that the mechanism of uptake is an endocytic process induced by virulent mycobacteria.
Similar articles
- Shigella infection of henle intestinal epithelial cells: role of the host cell.
Hale TL, Morris RE, Bonventre PF. Hale TL, et al. Infect Immun. 1979 Jun;24(3):887-94. doi: 10.1128/iai.24.3.887-894.1979. Infect Immun. 1979. PMID: 223988 Free PMC article. - Differences in uptake of mycobacteria by human monocytes: a role for complement.
Swartz RP, Naai D, Vogel CW, Yeager H Jr. Swartz RP, et al. Infect Immun. 1988 Sep;56(9):2223-7. doi: 10.1128/iai.56.9.2223-2227.1988. Infect Immun. 1988. PMID: 3137162 Free PMC article. - Fluorescent acid-fast microscopy for measuring phagocytosis of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum by Tetrahymena pyriformis and their intracellular growth.
Strahl ED, Gillaspy GE, Falkinham JO 3rd. Strahl ED, et al. Appl Environ Microbiol. 2001 Oct;67(10):4432-9. doi: 10.1128/AEM.67.10.4432-4439.2001. Appl Environ Microbiol. 2001. PMID: 11571139 Free PMC article. - Characterization of the intracellular survival of Mycobacterium avium ssp. paratuberculosis: phagosomal pH and fusogenicity in J774 macrophages compared with other mycobacteria.
Kuehnel MP, Goethe R, Habermann A, Mueller E, Rohde M, Griffiths G, Valentin-Weigand P. Kuehnel MP, et al. Cell Microbiol. 2001 Aug;3(8):551-66. doi: 10.1046/j.1462-5822.2001.00139.x. Cell Microbiol. 2001. PMID: 11488816 - Mycobacterium avium interaction with macrophages and intestinal epithelial cells.
Sangari FJ, Parker A, Bermudez LE. Sangari FJ, et al. Front Biosci. 1999 Jul 15;4:D582-8. doi: 10.2741/sangari. Front Biosci. 1999. PMID: 10417376 Review.
Cited by
- Mycobacterium tuberculosis invades and replicates within type II alveolar cells.
Bermudez LE, Goodman J. Bermudez LE, et al. Infect Immun. 1996 Apr;64(4):1400-6. doi: 10.1128/iai.64.4.1400-1406.1996. Infect Immun. 1996. PMID: 8606107 Free PMC article. - Mycobacterial disease, immunosuppression, and acquired immunodeficiency syndrome.
Collins FM. Collins FM. Clin Microbiol Rev. 1989 Oct;2(4):360-77. doi: 10.1128/CMR.2.4.360. Clin Microbiol Rev. 1989. PMID: 2680057 Free PMC article. Review. - Molecular characterization of the mycobacterial heparin-binding hemagglutinin, a mycobacterial adhesin.
Menozzi FD, Bischoff R, Fort E, Brennan MJ, Locht C. Menozzi FD, et al. Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12625-30. doi: 10.1073/pnas.95.21.12625. Proc Natl Acad Sci U S A. 1998. PMID: 9770536 Free PMC article. - Mycobacterium avium-intracellulare contamination of mammalian cell cultures.
Lelong-Rebel IH, Piemont Y, Fabre M, Rebel G. Lelong-Rebel IH, et al. In Vitro Cell Dev Biol Anim. 2009 Jan-Feb;45(1-2):75-90. doi: 10.1007/s11626-008-9143-8. Epub 2008 Oct 15. In Vitro Cell Dev Biol Anim. 2009. PMID: 18855078 - Epidemiology of infection by nontuberculous mycobacteria.
Falkinham JO 3rd. Falkinham JO 3rd. Clin Microbiol Rev. 1996 Apr;9(2):177-215. doi: 10.1128/CMR.9.2.177. Clin Microbiol Rev. 1996. PMID: 8964035 Free PMC article. Review. No abstract available.
References
- J Nutr. 1966 Nov;90(3):240-4 - PubMed
- Blood. 1968 Sep;32(3):423-35 - PubMed
- Nat New Biol. 1971 Aug 4;232(31):153-5 - PubMed
- J Clin Invest. 1972 Mar;51(3):615-24 - PubMed
- Am J Vet Res. 1972 Jul;33(7):1333-45 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources