Identification of immunogenic and antibody-binding membrane proteins of pathogenic Trichomonas vaginalis - PubMed (original) (raw)
Identification of immunogenic and antibody-binding membrane proteins of pathogenic Trichomonas vaginalis
J F Alderete. Infect Immun. 1983 Apr.
Abstract
Characterization of immunogenic Trichomonas vaginalis membrane proteins was accomplished by using extrinsically and intrinsically labeled organisms and a highly sensitive and specific radioimmunoprecipitation procedure. Intact motile trichomonads were compared with detergent extracts as a source of antigen in radioimmunoprecipitation experiments. Approximately 20 proteins accessible to antibody were identified and ranged in molecular weight from 200,000 to 20,000. Localization on the parasite surface of the highly immunogenic membrane proteins was attempted by using, as the indicator system, formaldehyde-fixed protein A-bearing Staphylococcus aureus pretreated with the various antiserum reagents and incubated with live, motile parasites. Also, indirect immunofluorescence with fluorescein isothiocyanate--anti-rabbit immunoglobulin was also employed after incubation of organisms with either control serum or antiserum from immunized rabbits or after treatment of trichomonads with the immunoglobulin G fraction from each respective serum. No immunoglobulin G antibody appeared to be directed at the anterior trichomonal flagella or the posterior axostyle, whereas strong fluorescence was detected throughout the rest of the T. vaginalis surface. The biological significance of these data is discussed.
Similar articles
- Antigen analysis of several pathogenic strains of Trichomonas vaginalis.
Alderete JF. Alderete JF. Infect Immun. 1983 Mar;39(3):1041-7. doi: 10.1128/iai.39.3.1041-1047.1983. Infect Immun. 1983. PMID: 6601618 Free PMC article. - Monoclonal antibody to a major surface glycoprotein immunogen differentiates isolates and subpopulations of Trichomonas vaginalis.
Alderete JF, Suprun-Brown L, Kasmala L. Alderete JF, et al. Infect Immun. 1986 Apr;52(1):70-5. doi: 10.1128/iai.52.1.70-75.1986. Infect Immun. 1986. PMID: 3514466 Free PMC article. - Heterogeneity of Trichomonas vaginalis and discrimination among trichomonal isolates and subpopulations with sera of patients and experimentally infected mice.
Alderete JF, Suprun-Brown L, Kasmala L, Smith J, Spence M. Alderete JF, et al. Infect Immun. 1985 Sep;49(3):463-8. doi: 10.1128/iai.49.3.463-468.1985. Infect Immun. 1985. PMID: 2411656 Free PMC article. - Humoral and T cell-mediated immune response against trichomoniasis.
Nemati M, Malla N, Yadav M, Khorramdelazad H, Jafarzadeh A. Nemati M, et al. Parasite Immunol. 2018 Mar;40(3). doi: 10.1111/pim.12510. Epub 2018 Jan 11. Parasite Immunol. 2018. PMID: 29266263 Review. - Strategies by which some pathogenic trichomonads integrate diverse signals in the decision-making process.
López LB, Braga MB, López JO, Arroyo R, Costa e Silva Filho F. López LB, et al. An Acad Bras Cienc. 2000 Jun;72(2):173-86. doi: 10.1590/s0001-37652000000200006. An Acad Bras Cienc. 2000. PMID: 10932116 Review.
Cited by
- Point-of-Care Diagnostic for Trichomonas vaginalis, the Most Prevalent, Non-Viral Sexually Transmitted Infection.
Alderete JF, Chan H. Alderete JF, et al. Pathogens. 2023 Jan 3;12(1):77. doi: 10.3390/pathogens12010077. Pathogens. 2023. PMID: 36678425 Free PMC article. - Epitopes within recombinant α-actinin protein is serodiagnostic target for Trichomonas vaginalis sexually transmitted infections.
Alderete JF. Alderete JF. Heliyon. 2017 Jan 30;3(1):e00237. doi: 10.1016/j.heliyon.2017.e00237. eCollection 2017 Jan. Heliyon. 2017. PMID: 28203642 Free PMC article. - Epitopes of the highly immunogenic Trichomonas vaginalis α-actinin are serodiagnostic targets for both women and men.
Neace CJ, Alderete JF. Neace CJ, et al. J Clin Microbiol. 2013 Aug;51(8):2483-90. doi: 10.1128/JCM.00582-13. Epub 2013 Apr 24. J Clin Microbiol. 2013. PMID: 23616456 Free PMC article. - Heterologous expression in Tritrichomonas foetus of functional Trichomonas vaginalis AP65 adhesin.
Kucknoor AS, Mundodi V, Alderete JF. Kucknoor AS, et al. BMC Mol Biol. 2005 Mar 4;6:5. doi: 10.1186/1471-2199-6-5. BMC Mol Biol. 2005. PMID: 15748280 Free PMC article. - Silencing the ap65 gene reduces adherence to vaginal epithelial cells by Trichomonas vaginalis.
Mundodi V, Kucknoor AS, Klumpp DJ, Chang TH, Alderete JF. Mundodi V, et al. Mol Microbiol. 2004 Aug;53(4):1099-108. doi: 10.1111/j.1365-2958.2004.04192.x. Mol Microbiol. 2004. PMID: 15306014 Free PMC article.
References
- J Protozool. 1968 Feb;15(1):176-84 - PubMed
- J Protozool. 1972 May;19(2):306-15 - PubMed
- Clin Obstet Gynecol. 1975 Mar;18(1):73-88 - PubMed
- Biochim Biophys Acta. 1975 Mar 25;382(3):276-85 - PubMed
- J Parasitol. 1976 Oct;62(5):826-8 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources