Information within the mature LamB protein necessary for localization to the outer membrane of E coli K12 - PubMed (original) (raw)
Information within the mature LamB protein necessary for localization to the outer membrane of E coli K12
S A Benson et al. Cell. 1983 Apr.
Abstract
It has been proposed that the efficient localization of the outer membrane protein LamB requires a functional signal sequence and at least two additional regions contained within the mature protein. We define these regions more precisely by deletion analysis, and we describe methods for cloning deleterious lacZ fusions onto high-copy-number plasmids and generating in-frame deletions. Analysis of the effects of a series of internal lamB deletions on the export of a LamB-LacZ hybrid protein and of the LamB protein itself indicates that necessary informational signal(s) required for localization lie at the amino-terminal end of the protein. In addition, our analysis indicates that there is a region of information close to or within the fusion joint of the largest lamB-lacZ fusion that increases the efficiency of the export process. A unique deletion that removes a protein segment from amino acid 70 to 200 appears to prevent proteolytic removal of the signal sequence. Nevertheless, the mutant protein is exported to the outer membrane.
Similar articles
- Intragenic regions required for LamB export.
Benson SA, Bremer E, Silhavy TJ. Benson SA, et al. Proc Natl Acad Sci U S A. 1984 Jun;81(12):3830-4. doi: 10.1073/pnas.81.12.3830. Proc Natl Acad Sci U S A. 1984. PMID: 6374667 Free PMC article. - The first 28 amino acids of mature LamB are required for rapid and efficient export from the cytoplasm.
Rasmussen BA, Silhavy TJ. Rasmussen BA, et al. Genes Dev. 1987 Apr;1(2):185-96. doi: 10.1101/gad.1.2.185. Genes Dev. 1987. PMID: 2824280 - Molecular components of the signal sequence that function in the initiation of protein export.
Emr SD, Silhavy TJ. Emr SD, et al. J Cell Biol. 1982 Dec;95(3):689-96. doi: 10.1083/jcb.95.3.689. J Cell Biol. 1982. PMID: 6759512 Free PMC article. - [Escherichia coli phage receptors. Minor porins and proteins participating in the specific transport as phage receptors].
Likhacheva NA, Sineokiĭ SP. Likhacheva NA, et al. Mol Gen Mikrobiol Virusol. 1989 Dec;(12):3-12. Mol Gen Mikrobiol Virusol. 1989. PMID: 2561376 Review. Russian. - Genetic analysis of protein export in Escherichia coli K12.
Benson SA, Hall MN, Silhavy TJ. Benson SA, et al. Annu Rev Biochem. 1985;54:101-34. doi: 10.1146/annurev.bi.54.070185.000533. Annu Rev Biochem. 1985. PMID: 3896116 Review. No abstract available.
Cited by
- Folding LacZ in the periplasm of Escherichia coli.
Dwyer RS, Malinverni JC, Boyd D, Beckwith J, Silhavy TJ. Dwyer RS, et al. J Bacteriol. 2014 Sep;196(18):3343-50. doi: 10.1128/JB.01843-14. Epub 2014 Jul 7. J Bacteriol. 2014. PMID: 25002543 Free PMC article. - Lethal mutations in the structural gene of an outer membrane protein (OmpA) of Escherichia coli K12.
Freudl R, Braun G, Hindennach I, Henning U. Freudl R, et al. Mol Gen Genet. 1985;201(1):76-81. doi: 10.1007/BF00397989. Mol Gen Genet. 1985. PMID: 2997584 - Mutation prlF1 relieves the lethality associated with export of beta-galactosidase hybrid proteins in Escherichia coli.
Kiino DR, Silhavy TJ. Kiino DR, et al. J Bacteriol. 1984 Jun;158(3):878-83. doi: 10.1128/jb.158.3.878-883.1984. J Bacteriol. 1984. PMID: 6233268 Free PMC article. - Intracellular targeting and import of an F1-ATPase beta-subunit-beta-galactosidase hybrid protein into yeast mitochondria.
Douglas MG, Geller BL, Emr SD. Douglas MG, et al. Proc Natl Acad Sci U S A. 1984 Jul;81(13):3983-7. doi: 10.1073/pnas.81.13.3983. Proc Natl Acad Sci U S A. 1984. PMID: 6330727 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources