In vivo and in vitro observations on laminin production by Schwann cells - PubMed (original) (raw)

In vivo and in vitro observations on laminin production by Schwann cells

C J Cornbrooks et al. Proc Natl Acad Sci U S A. 1983 Jun.

Abstract

Recent reports on the dystrophic mouse mutant suggest that the prominent extracellular matrix component of peripheral nerve tissues plays an important role in peripheral nerve development. We have examined the disposition of two prominent extracellular matrix components, fibronectin and laminin, both in mature peripheral nerve in vivo and in an in vitro system that allows study of Schwann cells in various functional states. In frozen sections of whole nerve, staining with antibodies to fibronectin and laminin shows that fibronectin stains throughout the endoneurium while laminin staining is restricted to regions known to contain basal lamina, particularly the basal lamina of each ensheathing Schwann cell. Tissue culture studies indicate that fibronectin staining at the light microscopic level is a reliable marker for fibroblasts (and not Schwann cells) in culture; conversely, antibodies to laminin stain components related to the Schwann cell surface but not components related to fibroblasts. Unexpectedly, Schwann cells in culture produce laminin at all stages in development, whether in contact with axons or not. As Schwann cells in culture begin to ensheathe axons, punctate regions of laminin on their surfaces become confluent. After ensheathment is completed, a continuous line of staining is found in the region of the Schwann cell basal lamina. It has been established that Schwann cells produce a basal lamina only when in contact with axons. Therefore, the production of laminin appears to be necessary but not sufficient for basal lamina formation. The constancy of laminin production by Schwann cells provides a reliable basis for distinguishing between Schwann cells and fibroblasts in tissue culture.

PubMed Disclaimer

Cited by

Differential expression of laminin chains and receptor (LBP-32) in fetal and neoplastic hepatocytes compared to normal adult hepatocytes in vivo and in culture.
Rescan PY, Clément B, Yamada Y, Segui-Real B, Baffet G, Guguen-Guillouzo C, Guillouzo A. Rescan PY, et al. Am J Pathol. 1990 Sep;137(3):701-9. Am J Pathol. 1990. PMID: 2144711 Free PMC article.
Sequence of the cDNA encoding the laminin B1 chain reveals a multidomain protein containing cysteine-rich repeats.
Sasaki M, Kato S, Kohno K, Martin GR, Yamada Y. Sasaki M, et al. Proc Natl Acad Sci U S A. 1987 Feb;84(4):935-9. doi: 10.1073/pnas.84.4.935. Proc Natl Acad Sci U S A. 1987. PMID: 3493487 Free PMC article.
Type 1 neurofibromatosis: selective expression of extracellular matrix genes by Schwann cells, perineurial cells, and fibroblasts in mixed cultures.
Jaakkola S, Peltonen J, Riccardi V, Chu ML, Uitto J. Jaakkola S, et al. J Clin Invest. 1989 Jul;84(1):253-61. doi: 10.1172/JCI114148. J Clin Invest. 1989. PMID: 2500456 Free PMC article.

References

1. Eur J Biochem. 1981 Sep;119(1):189-97 - PubMed
1. J Cell Biol. 1982 May;93(2):442-51 - PubMed
1. Dev Biol. 1982 Aug;92(2):449-60 - PubMed
1. Dev Biol. 1982 Oct;93(2):344-54 - PubMed
1. Brain Res. 1973 Jul 27;57(2):261-76 - PubMed

In vivo and in vitro observations on laminin production by Schwann cells - PubMed (original) (raw)