Sequence-specific interaction of R17 coat protein with its ribonucleic acid binding site - PubMed (original) (raw)
Sequence-specific interaction of R17 coat protein with its ribonucleic acid binding site
J Carey et al. Biochemistry. 1983.
Abstract
The interaction between phage R17 coat protein and its RNA binding site for translational repression was studied as an example of a sequence-specific RNA--protein interaction. Nuclease protection and selection experiments define the binding site to about 20 contiguous nucleotides which form a hairpin. A nitrocellulose filter retention assay is used to show that the binding between the coat protein and a synthetic 21-nucleotide RNA fragment conforms to a simple bimolecular reaction. Unit stoichiometry and a Kd of about 1 nM are obtained at 2 degrees C in buffer containing 0.19 M salt. The interaction is highly sequence specific since a variety of RNAs failed to compete with the 21-nucleotide fragment for coat protein binding.
Similar articles
- Interaction of R17 coat protein with its RNA binding site for translational repression.
Uhlenbeck OC, Carey J, Romaniuk PJ, Lowary PT, Beckett D. Uhlenbeck OC, et al. J Biomol Struct Dyn. 1983 Oct;1(2):539-52. doi: 10.1080/07391102.1983.10507460. J Biomol Struct Dyn. 1983. PMID: 6401118 Review. - Interaction of R17 coat protein with synthetic variants of its ribonucleic acid binding site.
Carey J, Lowary PT, Uhlenbeck OC. Carey J, et al. Biochemistry. 1983 Sep 27;22(20):4723-30. doi: 10.1021/bi00289a017. Biochemistry. 1983. PMID: 6626527 - Kinetic and thermodynamic characterization of the R17 coat protein-ribonucleic acid interaction.
Carey J, Uhlenbeck OC. Carey J, et al. Biochemistry. 1983 May 24;22(11):2610-5. doi: 10.1021/bi00280a003. Biochemistry. 1983. PMID: 6347248 - RNA binding site of R17 coat protein.
Romaniuk PJ, Lowary P, Wu HN, Stormo G, Uhlenbeck OC. Romaniuk PJ, et al. Biochemistry. 1987 Mar 24;26(6):1563-8. doi: 10.1021/bi00380a011. Biochemistry. 1987. PMID: 3297131 - Specific interaction between RNA phage coat proteins and RNA.
Witherell GW, Gott JM, Uhlenbeck OC. Witherell GW, et al. Prog Nucleic Acid Res Mol Biol. 1991;40:185-220. doi: 10.1016/s0079-6603(08)60842-9. Prog Nucleic Acid Res Mol Biol. 1991. PMID: 2031083 Review. No abstract available.
Cited by
- Identifying proteins that bind to specific RNAs - focus on simple repeat expansion diseases.
Jazurek M, Ciesiolka A, Starega-Roslan J, Bilinska K, Krzyzosiak WJ. Jazurek M, et al. Nucleic Acids Res. 2016 Nov 2;44(19):9050-9070. doi: 10.1093/nar/gkw803. Epub 2016 Sep 12. Nucleic Acids Res. 2016. PMID: 27625393 Free PMC article. Review. - Illuminating RNA Biology: Tools for Imaging RNA in Live Mammalian Cells.
Braselmann E, Rathbun C, Richards EM, Palmer AE. Braselmann E, et al. Cell Chem Biol. 2020 Aug 20;27(8):891-903. doi: 10.1016/j.chembiol.2020.06.010. Epub 2020 Jul 7. Cell Chem Biol. 2020. PMID: 32640188 Free PMC article. Review. - Characteristics of the interaction of the ferritin repressor protein with the iron-responsive element.
Swenson GR, Patino MM, Beck MM, Gaffield L, Walden WE. Swenson GR, et al. Biol Met. 1991;4(1):48-55. doi: 10.1007/BF01135557. Biol Met. 1991. PMID: 1854587 - Embryonic lethal abnormal vision-like RNA-binding proteins regulate neurite outgrowth and tau expression in PC12 cells.
Aranda-Abreu GE, Behar L, Chung S, Furneaux H, Ginzburg I. Aranda-Abreu GE, et al. J Neurosci. 1999 Aug 15;19(16):6907-17. doi: 10.1523/JNEUROSCI.19-16-06907.1999. J Neurosci. 1999. PMID: 10436048 Free PMC article. - An optimized streptavidin-binding RNA aptamer for purification of ribonucleoprotein complexes identifies novel ARE-binding proteins.
Leppek K, Stoecklin G. Leppek K, et al. Nucleic Acids Res. 2014 Jan;42(2):e13. doi: 10.1093/nar/gkt956. Epub 2013 Oct 23. Nucleic Acids Res. 2014. PMID: 24157833 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources