Interchangeability of repressors for the control of the uxu and uid operons in E. coli K12 - PubMed (original) (raw)

Comparative Study

Interchangeability of repressors for the control of the uxu and uid operons in E. coli K12

P Ritzenthaler et al. Mol Gen Genet. 1983.

Abstract

The uidA and uxuAB operons are each under the dual control of two repressor molecules: the uidR and uxuR encoded repressors negatively control the uidA operon whereas the uxuAB operon is regulated by the uxuR and exuR gene products. Plasmids overproducing the regulatory molecules encoded by exuR, uxuR, or uidR were used to investigate the regulation of these two operons. Large amounts of either exuR or uxuR repressor caused a complete repression of the uxuAB operon in exuR and uxuR double-deleted mutants, suggesting that the two repressors, when they are overproduced, are totally interchangeable for the control of the uxuAB operon. In contrast, the UxuR molecule has no effect on the other exu regulon operons controlled by the exuR gene product. The uidR and uxuR gene products appear to be partially interchangeable for the regulation of the uidA gene since addition of multicopy plasmids bearing uidR+, in uxuR deleted mutant strains, only partially suppresses the derepression of the uidA gene. Inversely, multicopies of uidR weakly reduced the synthesis of the uxuB gene product in uxuR derepressed mutants. The restrictions placed by these phenomena on the formulation of the mechanism of cooperation between the two repressor molecules for repressing the uxuAB and uidA operons are discussed.

PubMed Disclaimer

Cited by

Metabolically-targeted dCas9 expression in bacteria.
Pellegrino GM, Browne TS, Sharath K, Bari KA, Vancuren SJ, Allen-Vercoe E, Gloor GB, Edgell DR. Pellegrino GM, et al. Nucleic Acids Res. 2023 Jan 25;51(2):982-996. doi: 10.1093/nar/gkac1248. Nucleic Acids Res. 2023. PMID: 36629257 Free PMC article.
Short-Term Adaptation Modulates Anaerobic Metabolic Flux to Succinate by Activating ExuT, a Novel D-Glucose Transporter in Escherichia coli.
Kim HJ, Jeong H, Lee SJ. Kim HJ, et al. Front Microbiol. 2020 Jan 23;11:27. doi: 10.3389/fmicb.2020.00027. eCollection 2020. Front Microbiol. 2020. PMID: 32038601 Free PMC article.
Structural basis for the regulation of β-glucuronidase expression by human gut Enterobacteriaceae.
Little MS, Pellock SJ, Walton WG, Tripathy A, Redinbo MR. Little MS, et al. Proc Natl Acad Sci U S A. 2018 Jan 9;115(2):E152-E161. doi: 10.1073/pnas.1716241115. Epub 2017 Dec 21. Proc Natl Acad Sci U S A. 2018. PMID: 29269393 Free PMC article.
Engineering transcription factors with novel DNA-binding specificity using comparative genomics.
Desai TA, Rodionov DA, Gelfand MS, Alm EJ, Rao CV. Desai TA, et al. Nucleic Acids Res. 2009 May;37(8):2493-503. doi: 10.1093/nar/gkp079. Epub 2009 Mar 5. Nucleic Acids Res. 2009. PMID: 19264798 Free PMC article.
Genetic analysis of uxuR and exuR genes: evidence for ExuR and UxuR monomer repressors interactions.
Ritzenthaler P, Blanco C, Mata-Gilsinger M. Ritzenthaler P, et al. Mol Gen Genet. 1985;199(3):507-11. doi: 10.1007/BF00330766. Mol Gen Genet. 1985. PMID: 3929016

References

1. J Bacteriol. 1976 Jul;127(1):418-32 - PubMed
1. J Bacteriol. 1981 Jan;145(1):181-90 - PubMed
1. J Bacteriol. 1973 Nov;116(2):1064-6 - PubMed
1. J Bacteriol. 1981 Jan;145(1):211-20 - PubMed
1. J Bacteriol. 1982 Jun;150(3):1040-7 - PubMed

Interchangeability of repressors for the control of the uxu and uid operons in E. coli K12 - PubMed (original) (raw)