Cell-specific expression controlled by the 5'-flanking region of insulin and chymotrypsin genes - PubMed (original) (raw)

. 1983 Dec;306(5943):557-61.

doi: 10.1038/306557a0.

• PMID: 6358900
• DOI: 10.1038/306557a0

Cell-specific expression controlled by the 5'-flanking region of insulin and chymotrypsin genes

M D Walker et al. Nature. 1983 Dec.

Abstract

DNA sequences containing the 5'-flanking regions of the insulin and chymotrypsin genes were linked to the coding sequence of the chloramphenicol acetyltransferase (CAT) gene. The insulin gene recombinant elicits preferential expression of CAT activity when introduced into cells producing insulin; similarly, the chymotrypsin gene recombinant elicits preferential expression in chymotrypsin-producing cells. Sequences located upstream of previously defined transcriptional control elements are essential for efficient expression in both cases.

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