Cellular location of heat-labile enterotoxin in Escherichia coli - PubMed (original) (raw)

Cellular location of heat-labile enterotoxin in Escherichia coli

T R Hirst et al. J Bacteriol. 1984 Feb.

Abstract

We demonstrated that both the A and B subunits of heat-labile enterotoxin from Escherichia coli are located in the periplasm. The toxin was shown to form aggregates in Tris-EDTA buffers which are routinely used for isolating membranes. The aggregates pellet upon centrifugation, and this may explain why several previous investigators have concluded that enterotoxin is associated with membranes.

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