Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces - PubMed (original) (raw)
Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces
J V Kilmartin et al. J Cell Biol. 1984 Mar.
Abstract
The distribution of actin and tubulin during the cell cycle of the budding yeast Saccharomyces was mapped by immunofluorescence using fixed cells from which the walls had been removed by digestion. The intranuclear mitotic spindle was shown clearly by staining with a monoclonal antitubulin; the presence of extensive bundles of cytoplasmic microtubules is reported. In cells containing short spindles still entirely within the mother cells, one of the bundles of cytoplasmic microtubules nearly always extended to (or into) the bud. Two independent reagents (anti-yeast actin and fluorescent phalloidin) revealed an unusual distribution of actin: it was present as a set of cortical dots or patches and also as distinct fibers that were presumably bundles of actin filaments. Double labeling showed that at no stage in the cell cycle do the distributions of actin and tubulin coincide for any significant length, and, in particular, that the mitotic spindle did not stain detectably for actin. However, both microtubule and actin staining patterns change in a characteristic way during the cell cycle. In particular, the actin dots clustered in rings about the bases of very small buds and at the sites on unbudded cells at which bud emergence was apparently imminent. Later in the budding cycle, the actin dots were present largely in the buds and, in many strains, primarily at the tips of these buds. At about the time of cytokinesis the actin dots clustered in the neck region between the separating cells. These aspects of actin distribution suggest that it may have a role in the localized deposition of new cell wall material.
Similar articles
- Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic-mutant Saccharomyces cerevisiae.
Adams AE, Pringle JR. Adams AE, et al. J Cell Biol. 1984 Mar;98(3):934-45. doi: 10.1083/jcb.98.3.934. J Cell Biol. 1984. PMID: 6365931 Free PMC article. - Microtubules and actin cytoskeleton in Cryptococcus neoformans compared with ascomycetous budding and fission yeasts.
Kopecká M, Gabriel M, Takeo K, Yamaguchi M, Svoboda A, Ohkusu M, Hata K, Yoshida S. Kopecká M, et al. Eur J Cell Biol. 2001 Apr;80(4):303-11. doi: 10.1078/0171-9335-00157. Eur J Cell Biol. 2001. PMID: 11370745 - Studies concerning the temporal and genetic control of cell polarity in Saccharomyces cerevisiae.
Snyder M, Gehrung S, Page BD. Snyder M, et al. J Cell Biol. 1991 Aug;114(3):515-32. doi: 10.1083/jcb.114.3.515. J Cell Biol. 1991. PMID: 1860883 Free PMC article. - The cytoskeleton of Saccharomyces cerevisiae.
Barnes G, Drubin DG, Stearns T. Barnes G, et al. Curr Opin Cell Biol. 1990 Feb;2(1):109-15. doi: 10.1016/s0955-0674(05)80040-7. Curr Opin Cell Biol. 1990. PMID: 2183834 Review. No abstract available. - Cytoskeletal reorganization during process of apoptosis induced by cytostatic drugs in K-562 and HL-60 leukemia cell lines.
Grzanka A, Grzanka D, Orlikowska M. Grzanka A, et al. Biochem Pharmacol. 2003 Oct 15;66(8):1611-7. doi: 10.1016/s0006-2952(03)00532-x. Biochem Pharmacol. 2003. PMID: 14555241 Review.
Cited by
- The conserved protein adaptors CALM/AP180 and FCHo1/2 cooperatively recruit Eps15 to promote the initiation of clathrin-mediated endocytosis in yeast.
Sun Y, Yeam A, Kuo J, Iwamoto Y, Hu G, Drubin DG. Sun Y, et al. PLoS Biol. 2024 Sep 24;22(9):e3002833. doi: 10.1371/journal.pbio.3002833. eCollection 2024 Sep. PLoS Biol. 2024. PMID: 39316607 Free PMC article. - Improved tools for live imaging of F-actin structures in yeast.
Wirshing ACE, Goode BL. Wirshing ACE, et al. Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi: 10.1091/mbc.E24-05-0212-T. Epub 2024 Jul 18. Mol Biol Cell. 2024. PMID: 39024291 Free PMC article. - Purification of Human Cytoplasmic Actins From Saccharomyces cerevisiae.
Haarer BK, Amberg DC, Henty-Ridilla JL. Haarer BK, et al. Bio Protoc. 2023 Dec 5;13(23):e4894. doi: 10.21769/BioProtoc.4894. eCollection 2023 Dec 5. Bio Protoc. 2023. PMID: 38094253 Free PMC article. - Yeast Rim11 kinase responds to glutathione-induced stress by regulating the transcription of phospholipid biosynthetic genes.
Yasukawa T, Iwama R, Yamasaki Y, Masuo N, Noda Y. Yasukawa T, et al. Mol Biol Cell. 2024 Jan 1;35(1):ar8. doi: 10.1091/mbc.E23-03-0116. Epub 2023 Nov 8. Mol Biol Cell. 2024. PMID: 37938929 Free PMC article. - A dynamic actin cytoskeleton is required to prevent constitutive VDAC-dependent MAPK signalling and aberrant lipid homeostasis.
Davis J, Meyer T, Smolnig M, Smethurst DGJ, Neuhaus L, Heyden J, Broeskamp F, Edrich ESM, Knittelfelder O, Kolb D, Haar TV, Gourlay CW, Rockenfeller P. Davis J, et al. iScience. 2023 Aug 2;26(9):107539. doi: 10.1016/j.isci.2023.107539. eCollection 2023 Sep 15. iScience. 2023. PMID: 37636069 Free PMC article.
References
- Proc Natl Acad Sci U S A. 1979 Oct;76(10):4951-5 - PubMed
- Cold Spring Harb Symp Quant Biol. 1974;38:123-31 - PubMed
- Anal Biochem. 1973 Sep;55(1):328-30 - PubMed
- Nature. 1970 Aug 15;227(5259):680-5 - PubMed
- J Cell Biol. 1982 Mar;92(3):674-86 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases