Relationship of lipoamide dehydrogenases from Pseudomonas putida to other FAD-linked dehydrogenases - PubMed (original) (raw)

Comparative Study

. 1984 Mar 26;168(2):265-70.

doi: 10.1016/0014-5793(84)80259-8.

Free article

Comparative Study

Relationship of lipoamide dehydrogenases from Pseudomonas putida to other FAD-linked dehydrogenases

R Delaney et al. FEBS Lett. 1984.

Free article

Abstract

Pseudomonas putida produces two lipoamide dehydrogenases, LPD-glc and LPD-val. LPD-val is specifically required as the lipoamide dehydrogenase of branched-chain keto acid dehydrogenase and LPD-glc fulfills all other requirements for lipoamide dehydrogenase. Both proteins are dimers with one FAD per subunit. LPD-glc has an absorption maximum at 455 nm, but LPD-val has a maximum at 460 nm. Comparison of amino acid compositions revealed that LPD-glc was more closely related to Escherichia coli and pig heart lipoamide dehydrogenase than to LPD-val. LPD-val did not appear to be closely related to any of the proteins compared with the possible exception of mercuric reductase.

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