Synaptonemal complex-associated centromeres and recombination nodules in plant meiocytes prepared by an improved surface-spreading technique - PubMed (original) (raw)
Synaptonemal complex-associated centromeres and recombination nodules in plant meiocytes prepared by an improved surface-spreading technique
S M Albini et al. Exp Cell Res. 1984 Dec.
Abstract
An improved method of preparing two-dimensional surface spreads of plant synaptonemal complexes (SCs) is described. This technique produces clear preparations of SCs and, in addition, consistently reveals both centromeres and recombination nodules (RNs) in PTA-stained preparations viewed by electron microscopy. A preliminary study of RN number and distribution in Allium fistulosum indicates that they faithfully reflect the positions of cross-over exchange events as revealed by chiasmata.
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