HeN mice - PubMed (original) (raw)

Difference in susceptibility to gram-negative urinary tract infection between C3H/HeJ and C3H/HeN mice

L Hagberg et al. Infect Immun. 1984 Dec.

Abstract

The difference in susceptibility to urinary tract infection between C3H/HeJ and C3H/HeN mice was tested for with gram-negative strains differing in lipopolysaccharide composition. Recently, impaired clearance of Escherichia coli from the kidney of C3H/HeJ compared to C3H/HeN mice was shown to be correlated with the LPS low responsiveness. In this study, a difference in clearance from the kidneys of C3H/HeJ and C3H/HeN mice was found only with lipopolysaccharide-containing bacteria. Gram-positive bacteria, e.g., Staphylococcus saprophyticus and Streptococcus agalactiae, were recovered in essentially equal numbers from the kidneys of mice of both strains. In contrast, of the lipopolysaccharide-containing strains used, all persisted in higher numbers in the kidneys of C3H/HeJ mice than in the kidneys of C3H/HeN mice. Variations in the O side chain did not eliminate this difference. E. coli Hu734 O75+K5+ and the rfb- mutant O75-K5+ remained in similar numbers in C3H/HeJ mice, although O75-K5+ was eliminated more rapidly in C3H/HeN mice. The core structure did not affect the differential persistence in the two mouse strains. The rfb mutants with R1-R4 cores were eliminated after 24 h from the C3H/HeN mice, but remained in significant numbers in the kidneys of C3H/HeJ mice. Even the Re mutant of Salmonella minnesota persisted in low numbers in C3H/HeJ mice. The relative bacterial recovery from either mouse strain was related to the overall virulence of the infecting bacterial strain, but the difference between C3H/HeJ and C3H/HeN mice was associated with responsiveness to parts of lipopolysaccharide common to the bacterial strains tested.

PubMed Disclaimer

Cited by

Genetic control of the variable innate immune response to asymptomatic bacteriuria.
Grönberg-Hernández J, Sundén F, Connolly J, Svanborg C, Wullt B. Grönberg-Hernández J, et al. PLoS One. 2011;6(11):e28289. doi: 10.1371/journal.pone.0028289. Epub 2011 Nov 28. PLoS One. 2011. PMID: 22140570 Free PMC article.
The CTX-M-15-producing Escherichia coli clone O25b: H4-ST131 has high intestine colonization and urinary tract infection abilities.
Vimont S, Boyd A, Bleibtreu A, Bens M, Goujon JM, Garry L, Clermont O, Denamur E, Arlet G, Vandewalle A. Vimont S, et al. PLoS One. 2012;7(9):e46547. doi: 10.1371/journal.pone.0046547. Epub 2012 Sep 28. PLoS One. 2012. PMID: 23029548 Free PMC article.
TLR4-initiated and cAMP-mediated abrogation of bacterial invasion of the bladder.
Song J, Bishop BL, Li G, Duncan MJ, Abraham SN. Song J, et al. Cell Host Microbe. 2007 Jun 14;1(4):287-98. doi: 10.1016/j.chom.2007.05.007. Cell Host Microbe. 2007. PMID: 17710226 Free PMC article.
Association between TLR4 polymorphisms (896 A>G, 1196 C>T, - 2570 A>G, - 2081 G>A) and virulence factors in uropathogenic Escherichia coli.
Castro-Alarcón N, Rodríguez-García R, Ruíz-Rosas M, Muñoz-Valle JF, Guzmán-Guzmán IP, Parra-Rojas I, Vázquez-Villamar M. Castro-Alarcón N, et al. Clin Exp Med. 2019 Feb;19(1):105-113. doi: 10.1007/s10238-018-0527-0. Epub 2018 Sep 15. Clin Exp Med. 2019. PMID: 30220001
Bad bugs and beleaguered bladders: interplay between uropathogenic Escherichia coli and innate host defenses.
Mulvey MA, Schilling JD, Martinez JJ, Hultgren SJ. Mulvey MA, et al. Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8829-35. doi: 10.1073/pnas.97.16.8829. Proc Natl Acad Sci U S A. 2000. PMID: 10922042 Free PMC article. Review.

References

1. Eur J Biochem. 1974 Feb 15;42(1):303-9 - PubMed
1. J Immunol. 1980 Jan;124(1):20-4 - PubMed
1. Eur J Biochem. 1969 Oct;10(3):501-10 - PubMed
1. J Immunol. 1977 Jun;118(6):2088-93 - PubMed
1. Infect Immun. 1983 Apr;40(1):265-72 - PubMed

Difference in susceptibility to gram-negative urinary tract infection between C3H/HeJ and C3H/HeN mice - PubMed (original) (raw)