Effects of extracellular calcium depletion on membrane topography and occluding junctions of mammary epithelial cells in culture - PubMed (original) (raw)
Effects of extracellular calcium depletion on membrane topography and occluding junctions of mammary epithelial cells in culture
D R Pitelka et al. J Cell Biol. 1983 Mar.
Abstract
Ca2+ dependence of occluding junction structure and permeability, well documented in explanted or cultured epithelial sheets, presumably reflects inherent control mechanisms. As an approach to identification of these mechanisms, we induced disassembly of zonulae occludentes in confluent monolayers of mouse mammary epithelial cells by exposure to low concentrations of the chelators, EGTA or sodium citrate. Stages in disassembly were monitored during treatment by phase-contrast microscopy and prepared for transmission and scanning electron microscopy. Cellular response included several events affecting occluding junctions: (a) Centripetal cytoplasmic contraction created tension on junction membranes and displaced intramembrane strands along lines determined by the axis of tension. (b) Destabilization of junction position, probably through increased membrane fluidity, augmented tension-induced movement of strands, resulting in fragmentation of the junction belt. (c) Active ruffling and retraction of freed peripheral membranes remodeled cell borders to produce many filopodia, distally attached by occluding-junction fragments to neighboring cell membranes. Filopodia generally persisted until mechanically ruptured, when endocytosis of the junction and adhering cytoplasmic bleb ensued. Junction disassembly thus resulted from mechanical tensions generated by initial centripetal contraction and subsequent peripheral cytoskeletal activity, combined with destabilization of the junction's intramembrane strand pattern.
Similar articles
- Mechanical tension induces lateral movement of intramembrane components of the tight junction: studies on mouse mammary cells in culture.
Pitelka DR, Taggart BN. Pitelka DR, et al. J Cell Biol. 1983 Mar;96(3):606-12. doi: 10.1083/jcb.96.3.606. J Cell Biol. 1983. PMID: 6682108 Free PMC article. - Evidence that a Ca2+ chelator and a calmodulin blocker interfere with the structure of inter-Sertoli junctions.
Franchi E, Camatini M. Franchi E, et al. Tissue Cell. 1985;17(1):13-25. doi: 10.1016/0040-8166(85)90012-6. Tissue Cell. 1985. PMID: 3923652 - Structure, biochemistry, and assembly of epithelial tight junctions.
Gumbiner B. Gumbiner B. Am J Physiol. 1987 Dec;253(6 Pt 1):C749-58. doi: 10.1152/ajpcell.1987.253.6.C749. Am J Physiol. 1987. PMID: 3322036 Review. - The blood-testis barrier and Sertoli cell junctions: structural considerations.
Pelletier RM, Byers SW. Pelletier RM, et al. Microsc Res Tech. 1992 Jan 1;20(1):3-33. doi: 10.1002/jemt.1070200104. Microsc Res Tech. 1992. PMID: 1611148 Review.
Cited by
- A genetically targeted sensor reveals spatial and temporal dynamics of acrosomal calcium and sperm acrosome exocytosis.
Cohen R, Mukai C, Nelson JL, Zenilman SS, Sosnicki DM, Travis AJ. Cohen R, et al. J Biol Chem. 2022 May;298(5):101868. doi: 10.1016/j.jbc.2022.101868. Epub 2022 Mar 27. J Biol Chem. 2022. PMID: 35346690 Free PMC article. - Tether-guided lamellipodia enable rapid wound healing.
Korkmazhan E, Kennard AS, Garzon-Coral C, Vasquez CG, Dunn AR. Korkmazhan E, et al. Biophys J. 2022 Mar 15;121(6):1029-1037. doi: 10.1016/j.bpj.2022.02.006. Epub 2022 Feb 12. Biophys J. 2022. PMID: 35167863 Free PMC article. - Invasion and trafficking of hypervirulent group B streptococci in polarized enterocytes.
De Gaetano GV, Lentini G, Galbo R, Coppolino F, Famà A, Teti G, Beninati C. De Gaetano GV, et al. PLoS One. 2021 Jun 15;16(6):e0253242. doi: 10.1371/journal.pone.0253242. eCollection 2021. PLoS One. 2021. PMID: 34129624 Free PMC article. - Imaging effects of hyperosmolality on individual tricellular junctions.
Huang K, Zhou L, Alanis K, Hou J, Baker LA. Huang K, et al. Chem Sci. 2019 Dec 11;11(5):1307-1315. doi: 10.1039/c9sc05114g. eCollection 2020 Feb 7. Chem Sci. 2019. PMID: 33209250 Free PMC article. - Rho Flares Repair Local Tight Junction Leaks.
Stephenson RE, Higashi T, Erofeev IS, Arnold TR, Leda M, Goryachev AB, Miller AL. Stephenson RE, et al. Dev Cell. 2019 Feb 25;48(4):445-459.e5. doi: 10.1016/j.devcel.2019.01.016. Epub 2019 Feb 14. Dev Cell. 2019. PMID: 30773490 Free PMC article.
References
- Science. 1973 Jun 1;180(4089):971 - PubMed
- J Cell Sci. 1973 Nov;13(3):763-86 - PubMed
- J Comp Neurol. 1973 Dec 15;152(4):317-25 - PubMed
- Dev Biol. 1973 Dec;35(2):302-17 - PubMed
- J Cell Biol. 1974 Jun;61(3):575-90 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous