Mouse sn-glycerol-3-phosphate dehydrogenase: molecular cloning and genetic mapping of a cDNA sequence - PubMed (original) (raw)

Mouse sn-glycerol-3-phosphate dehydrogenase: molecular cloning and genetic mapping of a cDNA sequence

L P Kozak et al. Proc Natl Acad Sci U S A. 1983 May.

Abstract

The isozymes of glycerol-3-phosphate dehydrogenase (GPDH; sn-glycerol-3-phosphate:NAD+2-oxidoreductase, EC 1.1.1.8) in tissues of the mouse are coded for by two structural genes, Gdc-1 and Gdc-2, located on chromosomes 15 and 9, respectively. In order to investigate the expression of these genes, we isolated a GPDH cDNA clone from a mRNA preparation isolated from brown adipose tissue. The GPDH cDNA clone was identified by colony hybridization and hybrid selection of a mRNA that was translated in vitro to produce immunoprecipitable GPDH protein. In blot analysis, the GPDH cDNA hybridized to a single mRNA species that migrated at the position of 23S ribosomal RNA. This GPDH cDNA clone was mapped to the Gdc-1 locus by identification of a restriction enzyme polymorphism present in genomic DNA isolated from Gdc-1 congeneic lines of mice.

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References

1. 1. J Biol Chem. 1974 Jan 25;249(2):476-81 - PubMed
2. 1. J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
3. 1. Arch Biochem Biophys. 1977 Mar;179(2):682-9 - PubMed
4. 1. J Biol Chem. 1979 Jan 25;254(2):273-5 - PubMed
5. 1. Biochemistry. 1973 Jul 31;12(16):3055-63 - PubMed

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