Immunohistochemical staining of human brain with monoclonal antibodies that identify lymphocytes, monocytes, and the Ia antigen - PubMed (original) (raw)
Immunohistochemical staining of human brain with monoclonal antibodies that identify lymphocytes, monocytes, and the Ia antigen
S L Hauser et al. J Neuroimmunol. 1983 Oct.
Abstract
Using immunoperoxidase histochemistry, human brain sections obtained at biopsy were labeled with monoclonal antibodies which identify human lymphocyte subsets, monocytes, and the Ia antigen. Staining of a population of cells in white matter was present with the anti-Ia and the anti-M1 (monocyte-associated) antibodies but not with any of the 8 monoclonal antibodies which react with human T-cell subsets (anti-T1, 3, 4, 5, 6, 8, 10 and 12). The Ia antigen was present on 1-2% of cells in white matter, and approximately 5% of cells in white matter were M1-positive. Ia-positive cells demonstrated a pattern of diffuse surface membrane staining, whereas the M1 antigen appeared to cluster at proximal cell processes. Definitive identification of these cells as microglial cells, astrocytes or oligodendrocytes was not possible. These findings demonstrate that: (1) cells which bear the Ia and M1 determinants can be found in histologically normal human white matter, and (2) human oligodendrocytes do not react with monoclonal antibodies (anti-T5 and anti-T8) that identify human suppressor/cytotoxic cells.
Similar articles
- Cytoplasmic as opposed to surface Ia antigens expressed on human peripheral blood lymphocytes and monocytes.
Hofman FM, Billing RJ, Parker JW, Taylor CR. Hofman FM, et al. Clin Exp Immunol. 1982 Aug;49(2):355-63. Clin Exp Immunol. 1982. PMID: 6181924 Free PMC article. - Location of T cell and major histocompatibility complex antigens in the human thymus.
Bhan AK, Reinherz EL, Poppema S, McCluskey RT, Schlossman SF. Bhan AK, et al. J Exp Med. 1980 Oct 1;152(4):771-82. doi: 10.1084/jem.152.4.771. J Exp Med. 1980. PMID: 6158549 Free PMC article. - Expression and synthesis of murine immune response-associated (Ia) antigens by brain cells.
Ting JP, Shigekawa BL, Linthicum DS, Weiner LP, Frelinger JA. Ting JP, et al. Proc Natl Acad Sci U S A. 1981 May;78(5):3170-4. doi: 10.1073/pnas.78.5.3170. Proc Natl Acad Sci U S A. 1981. PMID: 6166944 Free PMC article. - Human Ia-like antigens (Analysis of the tissue distribution and immunochemical profile with monoclonal antibodies).
Natali PG, Russo C, Apollonj C, Pellegrino MA, Ferrone S. Natali PG, et al. Ric Clin Lab. 1981 Oct-Dec;11(4):287-300. Ric Clin Lab. 1981. PMID: 7034133 Review.
Cited by
- The endothelium--astrocyte immune control system of the brain.
Fontana A, Fierz W. Fontana A, et al. Springer Semin Immunopathol. 1985;8(1-2):57-70. doi: 10.1007/BF00197247. Springer Semin Immunopathol. 1985. PMID: 3890237 Review. No abstract available. - Differential suppression of interferon-gamma-induced Ia antigen expression on cultured rat astroglia and microglia by second messengers.
Sasaki A, Levison SW, Ting JP. Sasaki A, et al. J Neuroimmunol. 1990 Sep-Oct;29(1-3):213-22. doi: 10.1016/0165-5728(90)90164-i. J Neuroimmunol. 1990. PMID: 2170439 Free PMC article. - Shared antigenic determinants between human hemopoietic cells and nervous tissues and tumors.
Budka H, Majdic O. Budka H, et al. Acta Neuropathol. 1985;67(1-2):58-66. doi: 10.1007/BF00688124. Acta Neuropathol. 1985. PMID: 2411097 - The immunobiology of human gliomas.
Piguet V, Diserens AC, Carrel S, Mach JP, de Tribolet N. Piguet V, et al. Springer Semin Immunopathol. 1985;8(1-2):111-27. doi: 10.1007/BF00197250. Springer Semin Immunopathol. 1985. PMID: 3890235 Review. No abstract available. - Pathogenesis of primary central nervous system lymphoma: invasion of malignant lymphoid cells into and within the brain parenchyme.
Aho R, Ekfors T, Haltia M, Kalimo H. Aho R, et al. Acta Neuropathol. 1993;86(1):71-6. doi: 10.1007/BF00454901. Acta Neuropathol. 1993. PMID: 8372643