Proteolytic inactivation of human factor VIII procoagulant protein by activated human protein C and its analogy with factor V - PubMed (original) (raw)
- PMID: 6419799
Free article
Proteolytic inactivation of human factor VIII procoagulant protein by activated human protein C and its analogy with factor V
C A Fulcher et al. Blood. 1984 Feb.
Free article
Abstract
Purified human factor VIII procoagulant protein (VIII:C) was treated with purified human activated protein C (APC) and the loss of VIII:C activity correlated with proteolysis of the VIII:C polypeptides. APC proteolyzed all VIII:C polypeptides with mol wt = 92,000 or greater, but not the doublet at mol wt = 79-80,000. These results and our previous thrombin activation studies of purified VIII:C, are analogous with similar studies of factor V and form the basis for the following hypothesis: activated VIII:C consists of heavy and light chain polypeptides [mol wt = 92,000 and mol wt = 79-80,000 (or 71-72,000), respectively] which are similar in Mr to the heavy and light chains of activated factor V. Thrombin activates VIII:C and V by generating these polypeptide chains from larger precursors and APC inactivates both molecules by cleavage at a site located in the heavy chain region of activated VIII:C and V.
Similar articles
- Human factor VIII procoagulant protein. Monoclonal antibodies define precursor-product relationships and functional epitopes.
Fulcher CA, Roberts JR, Holland LZ, Zimmerman TS. Fulcher CA, et al. J Clin Invest. 1985 Jul;76(1):117-24. doi: 10.1172/JCI111933. J Clin Invest. 1985. PMID: 2410456 Free PMC article. - Mechanisms that regulate the anticoagulant function of coagulation factor V.
Thorelli E. Thorelli E. Scand J Clin Lab Invest Suppl. 1999;229:19-26. doi: 10.1080/00365519950185913. Scand J Clin Lab Invest Suppl. 1999. PMID: 10097286 Review. - Isolation, subunit structure, and proteolytic modification of bovine factor VIII.
Legaz ME, Weinstein MJ, Heldebrant CM, Davie EW. Legaz ME, et al. Ann N Y Acad Sci. 1975 Jan 20;240:43-61. doi: 10.1111/j.1749-6632.1975.tb53321.x. Ann N Y Acad Sci. 1975. PMID: 122888 Review.
Cited by
- Evidence that a secondary binding and protecting site for factor VIII on von Willebrand factor is highly unlikely.
Layet S, Girma JP, Obert B, Peynaud-Debayle E, Bihoreau N, Meyer D. Layet S, et al. Biochem J. 1992 Feb 15;282 ( Pt 1)(Pt 1):129-37. doi: 10.1042/bj2820129. Biochem J. 1992. PMID: 1531749 Free PMC article. - Safety assessment of drotrecogin alfa (activated) in the treatment of adult patients with severe sepsis.
Bernard GR, Macias WL, Joyce DE, Williams MD, Bailey J, Vincent JL. Bernard GR, et al. Crit Care. 2003 Apr;7(2):155-63. doi: 10.1186/cc2167. Epub 2003 Feb 28. Crit Care. 2003. PMID: 12720562 Free PMC article. Review. - Recombinant human activated protein C ameliorates oleic acid-induced lung injury in awake sheep.
Waerhaug K, Kirov MY, Kuzkov VV, Kuklin VN, Bjertnaes LJ. Waerhaug K, et al. Crit Care. 2008;12(6):R146. doi: 10.1186/cc7128. Epub 2008 Nov 20. Crit Care. 2008. PMID: 19021914 Free PMC article. - Acute Traumatic Coagulopathy: The Value of Histone in Pediatric Trauma Patients.
Ulusoy E, Duman M, Çağlar A, Küme T, Er A, Akgül F, Çitlenbik H, Yılmaz D, Ören H. Ulusoy E, et al. Turk J Haematol. 2018 May 25;35(2):122-128. doi: 10.4274/tjh.2017.0444. Epub 2018 Mar 28. Turk J Haematol. 2018. PMID: 29589832 Free PMC article. - The haemostatic function of the vascular endothelial cell.
Bull HA, Machin SJ. Bull HA, et al. Blut. 1987 Aug;55(2):71-80. doi: 10.1007/BF00631776. Blut. 1987. PMID: 3111566 Review. No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources