Differentiation-dependent chromatin alterations precede and accompany transcription of immunoglobulin light chain genes - PubMed (original) (raw)
. 1984 Jul 10;259(13):8534-44.
- PMID: 6429143
Free article
Differentiation-dependent chromatin alterations precede and accompany transcription of immunoglobulin light chain genes
S M Rose et al. J Biol Chem. 1984.
Free article
Abstract
We have studied the nature of chromatin alterations along immunoglobulin light chain (IgL) genes during B cell development using cultured murine cell lines. Employing a chromatin fractionation procedure on micrococcal nuclease-treated nuclei, we demonstrate that transcriptionally active k IgL chromatin lacks a canonical nucleosomal repeat and exhibits a pronounced association with insoluble nuclear material but is processed by nuclease to a soluble nucleosomal component that apparently lacks histone H1 and is enriched in high mobility group proteins. Of particular significance, utilizing a variant plasmacytoma cell line that has transcriptionally inactivated one k allele via a promoter deletion, we demonstrate that transcription per se is not responsible for these novel alterations. Furthermore, we show that the chromatin encompassing germline (unrearranged) and transcriptionally silent lambda IgL alleles in k-producing plasmacytomas exhibit some of the same unusual properties that are displayed by k alleles. Finally, we demonstrate that these alterations only occur in cell lines of the lymphocyte lineage that have progressed past the early pre-B cell stage; when inactive, both k and lambda IgL genes possess typical nucleosomal packaging and co-fractionate with histone H1-containing chromatin. These findings lead us to propose a model that predicts B cell stage-specific alterations in IgL chromatin prior to gene rearrangement and transcription.
Similar articles
- Varigated chromatin structures of mouse ribosomal RNA genes.
Davis AH, Reudelhuber TL, Garrard WT. Davis AH, et al. J Mol Biol. 1983 Jun 15;167(1):133-55. doi: 10.1016/s0022-2836(83)80038-2. J Mol Biol. 1983. PMID: 6864798 - Transcribed chromatin exhibits an altered nucleosomal spacing.
Smith RD, Seale RL, Yu J. Smith RD, et al. Proc Natl Acad Sci U S A. 1983 Sep;80(18):5505-9. doi: 10.1073/pnas.80.18.5505. Proc Natl Acad Sci U S A. 1983. PMID: 6225120 Free PMC article. - Nucleosome disruption precedes transcription and is largely limited to the transcribed domain of globin genes in murine erythroleukemia cells.
Cohen RB, Sheffery M. Cohen RB, et al. J Mol Biol. 1985 Mar 5;182(1):109-29. doi: 10.1016/0022-2836(85)90031-2. J Mol Biol. 1985. PMID: 2582137 - Somatic diversification of the chicken immunoglobulin light-chain gene.
McCormack WT, Thompson CB. McCormack WT, et al. Adv Immunol. 1990;48:41-67. doi: 10.1016/s0065-2776(08)60751-8. Adv Immunol. 1990. PMID: 2112303 Review. - Structure of transcriptionally active chromatin.
Yaniv M, Cereghini S. Yaniv M, et al. CRC Crit Rev Biochem. 1986;21(1):1-26. doi: 10.3109/10409238609113607. CRC Crit Rev Biochem. 1986. PMID: 3015490 Review.
Cited by
- Evaluation of the Moonlighting Histone H3 Specific Protease (H3ase) Activity and the Dehydrogenase Activity of Glutamate Dehydrogenase (GDH).
Purohit JS, Singh M, Raghuvanshi Y, Syeda S, Chaturvedi MM. Purohit JS, et al. Cell Biochem Biophys. 2024 Mar;82(1):223-233. doi: 10.1007/s12013-023-01201-9. Epub 2023 Dec 1. Cell Biochem Biophys. 2024. PMID: 38040891 - Phosphorylation of distinct sites in MeCP2 modifies cofactor associations and the dynamics of transcriptional regulation.
Gonzales ML, Adams S, Dunaway KW, LaSalle JM. Gonzales ML, et al. Mol Cell Biol. 2012 Jul;32(14):2894-903. doi: 10.1128/MCB.06728-11. Epub 2012 May 21. Mol Cell Biol. 2012. PMID: 22615490 Free PMC article. - The protamine-like DNA-binding protein P6.9 epigenetically up-regulates Autographa californica multiple nucleopolyhedrovirus gene transcription in the late infection phase.
Peng Y, Li K, Pei RJ, Wu CC, Liang CY, Wang Y, Chen XW. Peng Y, et al. Virol Sin. 2012 Feb;27(1):57-68. doi: 10.1007/s12250-012-3229-x. Epub 2012 Jan 22. Virol Sin. 2012. PMID: 22270807 Free PMC article. - MeCP2 binds to nucleosome free (linker DNA) regions and to H3K9/H3K27 methylated nucleosomes in the brain.
Thambirajah AA, Ng MK, Frehlick LJ, Li A, Serpa JJ, Petrotchenko EV, Silva-Moreno B, Missiaen KK, Borchers CH, Adam Hall J, Mackie R, Lutz F, Gowen BE, Hendzel M, Georgel PT, Ausió J. Thambirajah AA, et al. Nucleic Acids Res. 2012 Apr;40(7):2884-97. doi: 10.1093/nar/gkr1066. Epub 2011 Dec 5. Nucleic Acids Res. 2012. PMID: 22144686 Free PMC article. - Histone H2A C-terminus regulates chromatin dynamics, remodeling, and histone H1 binding.
Vogler C, Huber C, Waldmann T, Ettig R, Braun L, Izzo A, Daujat S, Chassignet I, Lopez-Contreras AJ, Fernandez-Capetillo O, Dundr M, Rippe K, Längst G, Schneider R. Vogler C, et al. PLoS Genet. 2010 Dec 9;6(12):e1001234. doi: 10.1371/journal.pgen.1001234. PLoS Genet. 2010. PMID: 21170357 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Miscellaneous