An improved procedure for immunoelectron microscopy: ultrathin plastic embedding of immunolabeled ultrathin frozen sections - PubMed (original) (raw)
An improved procedure for immunoelectron microscopy: ultrathin plastic embedding of immunolabeled ultrathin frozen sections
G A Keller et al. Proc Natl Acad Sci U S A. 1984 Sep.
Abstract
Ultrathin frozen sections are ideal substrates with which to carry out immunolabeling experiments in electron microscopy. However, the ultrastructural delineation in positively stained frozen sections has not been as detailed as in conventionally osmium-stained and plastic-embedded sections. We now describe a simple technique in which immunolabeled ultrathin frozen sections are subsequently treated with osmium tetroxide, dehydrated, and then embedded in plastic by impregnation with a monomer to the thickness of the section, followed by polymerization of the monomer. By this technique ultrastructural definition as good as that of conventional plastic sections is achieved, while the high density and specificity of immunolabeling characteristic of ultrathin frozen sections are retained.
Similar articles
- Ultrathin frozen sections. I. Methods and ultrastructural preservation.
Bernhard W, Leduc EH. Bernhard W, et al. J Cell Biol. 1967 Sep;34(3):757-71. doi: 10.1083/jcb.34.3.757. J Cell Biol. 1967. PMID: 4167504 Free PMC article. - Immunoelectron microscopic labeling of immunoglobulin in plasma cells after osmium fixation and epoxy embedding.
Hearn SA, Silver MM, Sholdice JA. Hearn SA, et al. J Histochem Cytochem. 1985 Dec;33(12):1212-8. doi: 10.1177/33.12.3934258. J Histochem Cytochem. 1985. PMID: 3934258 - Application of microwave technology to the processing and immunolabeling of plastic-embedded and cryosections.
Rangell LK, Keller GA. Rangell LK, et al. J Histochem Cytochem. 2000 Aug;48(8):1153-9. doi: 10.1177/002215540004800812. J Histochem Cytochem. 2000. PMID: 10898808 - Light microscopic histochemistry on plastic sections.
Litwin JA. Litwin JA. Prog Histochem Cytochem. 1985;16(2):1-84. doi: 10.1016/s0079-6336(85)80001-2. Prog Histochem Cytochem. 1985. PMID: 2417278 Review. - Applications of immunogold labeling in ultrastructural pathology.
Zuber C, Fan J, Guhl B, Roth J. Zuber C, et al. Ultrastruct Pathol. 2005 May-Aug;29(3-4):319-30. doi: 10.1080/01913120590951310. Ultrastruct Pathol. 2005. PMID: 16036886 Review.
Cited by
- Identification of RSV Fusion Protein Interaction Domains on the Virus Receptor, Nucleolin.
Mastrangelo P, Chin AA, Tan S, Jeon AH, Ackerley CA, Siu KK, Lee JE, Hegele RG. Mastrangelo P, et al. Viruses. 2021 Feb 8;13(2):261. doi: 10.3390/v13020261. Viruses. 2021. PMID: 33567674 Free PMC article. - Identification of neuromuscular junctions by correlative confocal and transmission electron microscopy.
Modla S, Mendonca J, Czymmek KJ, Akins RE. Modla S, et al. J Neurosci Methods. 2010 Aug 30;191(2):158-65. doi: 10.1016/j.jneumeth.2010.06.015. Epub 2010 Jun 25. J Neurosci Methods. 2010. PMID: 20600319 Free PMC article. - Correct targeting of the bean storage protein phaseolin in the seeds of transformed tobacco.
Greenwood JS, Chrispeels MJ. Greenwood JS, et al. Plant Physiol. 1985 Sep;79(1):65-71. doi: 10.1104/pp.79.1.65. Plant Physiol. 1985. PMID: 16664403 Free PMC article.
References
- J Cell Biol. 1973 May;57(2):551-65 - PubMed
- J Cell Biol. 1983 Nov;97(5 Pt 1):1559-65 - PubMed
- J Cell Biol. 1981 Jun;89(3):653-65 - PubMed
- J Cell Biol. 1981 Dec;91(3 Pt 1):614-28 - PubMed
- Proc Natl Acad Sci U S A. 1981 Dec;78(12):7619-23 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources