Structural homology between Drosophila melanogaster and Escherichia coli acidic ribosomal proteins - PubMed (original) (raw)

Comparative Study

. 1984 Aug;22(7-8):749-67.

doi: 10.1007/BF00485858.

Comparative Study

Structural homology between Drosophila melanogaster and Escherichia coli acidic ribosomal proteins

W Y Chooi et al. Biochem Genet. 1984 Aug.

Abstract

Antibodies raised against Drosophila melanogaster ribosomal proteins (r-proteins) were used to examine possible structural relationships between eukaryotic and prokaryotic r-proteins. The antisera were raised against either groups of r-proteins or individually purified r-proteins. Two antisera showed a cross-reaction with total Escherichia coli r-proteins in Ouchterlony double immunodiffusion assays: an antiserum against the D. melanogaster small subunit protein S14 (anti-S14) and an antiserum against a group of D. melanogaster r-proteins (anti-TP80). The specificity of the antisera and the identity of the homologous E. coli r-proteins were characterized by using immunooverlay and immunoblot assays. These assays indicated that anti-S14 was highly specific for protein S14 and anti-TP80 was a multispecific serum that recognized several of the D. melanogaster ribosomal proteins. The E. coli protein homologous to D. melanogaster protein S14 was identified as E. coli protein S6. By adsorption of the anti-TP80 serum, we determined that D. melanogaster protein 7/8 is homologous to the acidic E. coli protein L7/L12. D. melanogaster acidic protein 13 was also shown to be immunologically related to D. melanogaster protein 7/8.

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References

    1. J Biol Chem. 1978 Feb 10;253(3):946-55 - PubMed
    1. J Biol Chem. 1976 Mar 25;251(6):1799-807 - PubMed
    1. Biochemistry. 1980 Apr 1;19(7):1425-33 - PubMed
    1. J Biol Chem. 1982 Aug 10;257(15):9189-97 - PubMed
    1. Immunochemistry. 1970 Nov;7(11):885-98 - PubMed

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