High molecular weight complex in human plasma between vitamin K-dependent protein S and complement component C4b-binding protein - PubMed (original) (raw)
High molecular weight complex in human plasma between vitamin K-dependent protein S and complement component C4b-binding protein
B Dahlbäck et al. Proc Natl Acad Sci U S A. 1981 Apr.
Abstract
Protein S, a recently described vitamin K-dependent plasma protein, is shown to exist in two forms in plasma--free protein and in complex with C4b-binding protein. C4b-binding protein is involved in the regulation of the rate of complement activation. A major proportion of C4b-binding protein in plasma is in complex with protein S. The complex is a major and previously unrecognized component of the group of plasma proteins that adsorbs to barium citrate. The complex dissociates in the presence of NaDodSO4, indicating that C4b-binding protein and protein S are held together by noncovalent bonds. Uncomplexed C4b-binding protein was purified from the supernatant after barium citrate adsorption. On NaDodSO4/polyacrylamide gels without reduction, it appeared to have a slightly faster migration rate than the C4b-binding protein dissociated from the complex with protein S. After reduction, the subunits of the two forms of C4b-binding protein appeared to have identical molecular weights. Furthermore, there is an equilibrium between free and bound protein S in plasma. The role of protein S in the complex is unknown.
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References
- Biochim Biophys Acta. 1971 Dec 28;251(3):363-9 - PubMed
- Annu Rev Biochem. 1980;49:765-811 - PubMed
- J Biol Chem. 1975 Aug 10;250(15):6125-33 - PubMed
- J Biol Chem. 1976 Jan 25;251(2):355-63 - PubMed
- Biochemistry. 1977 Feb 22;16(4):698-706 - PubMed
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