Xenopus hsp 70 genes are constitutively expressed in injected oocytes - PubMed (original) (raw)

Xenopus hsp 70 genes are constitutively expressed in injected oocytes

M Bienz. EMBO J. 1984 Nov.

Abstract

Xenopus heat-shock genes are transiently heat-inducible in somatic cells, but they are also subject to a long-term developmental control in oogenesis and early embryogenesis. In order to understand whether different genes or different promoter elements are involved in the two types of control, several genomic clones coding for Xenopus heat-shock proteins, hsp 70 and hsp 30, were isolated, characterised and tested for expression in oocytes and COS cells. Three isolated hsp 70 genes are nearly identical in their promoter and mRNA leader sequences, indicating that there is only one type of hsp 70 gene. These promoters contain a consensus sequence element (CT-GAA--TTC-AG) upstream of the TATA-box, which is presumably required for their transient heat-inducibility. The two isolated hsp 30 genes show 5'-flanking sequences similar to each other, except that one of them shows a homology disruption precisely around the consensus sequence element. The same gene contains a frameshift mutation in the protein coding part and, since it cannot be expressed after introduction into oocytes or COS cells, it is probably a pseudogene. The other hsp 30 gene is strongly heat-inducible in injected oocytes or transfected COS cells. In contrast, the hsp 70 genes are strongly heat-inducible in COS cells, but their expression is highly efficient in injected oocytes at the normal temperature and is not increased during heat shock. This represents correct cell type-specific regulation of a cloned reintroduced gene, since the endogenous hsp 70 genes are constitutively activated during oogenesis, leading to the accumulation of stored hsp 70 mRNA in oocytes.

PubMed Disclaimer

Similar articles

• A synthetic heat-shock promoter element confers heat-inducibility on the herpes simplex virus thymidine kinase gene.
  Pelham HR, Bienz M. Pelham HR, et al. EMBO J. 1982;1(11):1473-7. doi: 10.1002/j.1460-2075.1982.tb01340.x. EMBO J. 1982. PMID: 6327271 Free PMC article.
• Developmental control of the heat shock response in Xenopus.
  Bienz M. Bienz M. Proc Natl Acad Sci U S A. 1984 May;81(10):3138-42. doi: 10.1073/pnas.81.10.3138. Proc Natl Acad Sci U S A. 1984. PMID: 6203112 Free PMC article.
• Identification of a sequence element in the promoter of the Drosophila melanogaster hsp23 gene that is required for its heat activation.
  Mestril R, Rungger D, Schiller P, Voellmy R. Mestril R, et al. EMBO J. 1985 Nov;4(11):2971-6. doi: 10.1002/j.1460-2075.1985.tb04031.x. EMBO J. 1985. PMID: 2998770 Free PMC article.
• Expression of a Drosophila heat-shock protein in Xenopus oocytes: conserved and divergent regulatory signals.
  Bienz M, Pelham HR. Bienz M, et al. EMBO J. 1982;1(12):1583-8. doi: 10.1002/j.1460-2075.1982.tb01359.x. EMBO J. 1982. PMID: 6821336 Free PMC article.
• The use of the Xenopus oocyte as a model system to analyze the expression and function of eukaryotic heat shock proteins.
  Heikkila JJ, Kaldis A, Morrow G, Tanguay RM. Heikkila JJ, et al. Biotechnol Adv. 2007 Jul-Aug;25(4):385-95. doi: 10.1016/j.biotechadv.2007.03.003. Epub 2007 Mar 28. Biotechnol Adv. 2007. PMID: 17459646 Review.

Cited by

• Xenopus Y-box transcription factors: molecular cloning, functional analysis and developmental regulation.
  Tafuri SR, Wolffe AP. Tafuri SR, et al. Proc Natl Acad Sci U S A. 1990 Nov;87(22):9028-32. doi: 10.1073/pnas.87.22.9028. Proc Natl Acad Sci U S A. 1990. PMID: 2247479 Free PMC article.
• Cell-Nonautonomous Regulation of Proteostasis in Aging and Disease.
  Morimoto RI. Morimoto RI. Cold Spring Harb Perspect Biol. 2020 Apr 1;12(4):a034074. doi: 10.1101/cshperspect.a034074. Cold Spring Harb Perspect Biol. 2020. PMID: 30962274 Free PMC article. Review.
• Nuclear history of a pre-mRNA determines the translational activity of cytoplasmic mRNA.
  Matsumoto K, Wassarman KM, Wolffe AP. Matsumoto K, et al. EMBO J. 1998 Apr 1;17(7):2107-21. doi: 10.1093/emboj/17.7.2107. EMBO J. 1998. PMID: 9524132 Free PMC article.
• Cloning and characterization of a 70 kd heat shock cognate (hsc70) gene from the zebrafish (Danio rerio).
  Graser RT, Malnar-Dragojevic D, Vincek V. Graser RT, et al. Genetica. 1996-1997;98(3):273-6. doi: 10.1007/BF00057591. Genetica. 1996. PMID: 9204550
• Phylogenetic analysis of the stress-70 protein family.
  Rensing SA, Maier UG. Rensing SA, et al. J Mol Evol. 1994 Jul;39(1):80-6. doi: 10.1007/BF00178252. J Mol Evol. 1994. PMID: 7545947

References

1. 1. J Mol Biol. 1980 Oct 25;143(2):161-78 - PubMed
2. 1. Cell. 1984 Sep;38(2):391-8 - PubMed
3. 1. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7038-42 - PubMed
4. 1. Dev Biol. 1982 Jan;89(1):196-210 - PubMed
5. 1. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1776-80 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • Nature Publishing Group
  • PubMed Central

• Medical

  • MedlinePlus Health Information

• Miscellaneous

  • NCI CPTAC Assay Portal