A cytological map of the human X chromosome--evidence for non-random recombination - PubMed (original) (raw)

A cytological map of the human X chromosome--evidence for non-random recombination

D A Hartley et al. Nucleic Acids Res. 1984.

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Abstract

The cytological location of six cloned DNA sequences on the human X chromosome has been determined to a high resolution by direct hybridisation 'in situ' to metaphase chromosomes. Each locus has been identified using clones which also detect restriction fragment length polymorphisms by Southern hybridisation. The six loci identified are spaced along the chromosome from Xp22 to Xq28. By combining data obtained using this powerful sequence localisation technique with that from hybrid cell panels and from family studies, it is possible to compare physical and genetic distances, and to demonstrate that the frequency of reciprocal genetic exchange is not uniform along the chromosome length.

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