Murine hemopoietic colonies in culture containing normoblasts, macrophages, and megakaryocytes - PubMed (original) (raw)

Murine hemopoietic colonies in culture containing normoblasts, macrophages, and megakaryocytes

H Hara et al. Am J Hematol. 1978.

Abstract

Murine marrow cells, when incubated in methylcellulose culture in the presence of erythropoietin and conditioned medium for two weeks, produced large macroscopic bursts containing normoblasts, macrophages, and often megakaryocytes. The clonal nature of these mixed colonies was supported by linearity studies and analysis of the percentages of constituent cells in different plating conditions. Time course observations and studies of the effects of L-cell-conditioned medium revealed that colony-forming cells for the mixed colonies (CFU-mix) are at earlier stages of hemopoietic development than burst-forming units (BFU-E). The mean of the modal sedimentation velocities of CFU-mix was 3.4 mm/hr and was in close agreement with that reported for the spleen colony-forming units. Almost none of the CFU-mix was in a DNA synthetic phase as measured by short-term exposure to tritiated thymidine. These results strongly indicate that CFU-mix represent a population of pluripotent hemopoietic stem cells in murine marrow.

PubMed Disclaimer

Similar articles

• Erythropoietic precursors in mice under erythropoietic stimulation and suppression.
  Hara H, Ogawa M. Hara H, et al. Exp Hematol. 1977 Mar;5(2):141-8. Exp Hematol. 1977. PMID: 844518
• Colony formation in agar by adult bone marrow multipotential hemopoietic cells.
  Johnson GR. Johnson GR. J Cell Physiol. 1980 Jun;103(3):371-83. doi: 10.1002/jcp.1041030302. J Cell Physiol. 1980. PMID: 7400223
• Clonal origin of murine hemopoietic colonies with apparent restriction to granuclocyte-macrophage-megakaryocyte (GMM) differentiation.
  Nakahata T, Ogawa M. Nakahata T, et al. J Cell Physiol. 1982 Jun;111(3):239-46. doi: 10.1002/jcp.1041110304. J Cell Physiol. 1982. PMID: 7096452
• Clonal nature of pluripotent hemopoietic precursors in vitro (CFU-mix).
  Hara H, Noguchi K. Hara H, et al. Stem Cells (1981). 1981;1(1):53-60. Stem Cells (1981). 1981. PMID: 7348869
• Alternatives to classical mitosis in hemopoietic tissues of vertebrates.
  Engelbert VE. Engelbert VE. Int Rev Cytol. 1979;60:93-120. doi: 10.1016/s0074-7696(08)61260-7. Int Rev Cytol. 1979. PMID: 387645 Review. No abstract available.

Cited by

• Resolving the hematopoietic stem cell state by linking functional and molecular assays.
  Jassinskaja M, Gonka M, Kent DG. Jassinskaja M, et al. Blood. 2023 Aug 10;142(6):543-552. doi: 10.1182/blood.2022017864. Blood. 2023. PMID: 36735913 Free PMC article. Review.
• Myelo-lymphopoietic stem cells in human bone marrow.
  Fauser AA, Löhr GW. Fauser AA, et al. Blut. 1982 Sep;45(3):151-5. doi: 10.1007/BF00320799. Blut. 1982. PMID: 6981442 No abstract available.
• Human megakaryocytes. V. Changes in the phenotypic profile of differentiating megakaryocytes.
  Levene RB, Lamaziere JM, Broxmeyer HE, Lu L, Rabellino EM. Levene RB, et al. J Exp Med. 1985 Mar 1;161(3):457-74. doi: 10.1084/jem.161.3.457. J Exp Med. 1985. PMID: 3973535 Free PMC article.
• Polyploid megakaryocytes develop randomly from a multicompartmental system of committed progenitors.
  Paulus JM, Prenant M, Deschamps JF, Henry-Amar M. Paulus JM, et al. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4410-4. doi: 10.1073/pnas.79.14.4410. Proc Natl Acad Sci U S A. 1982. PMID: 6956870 Free PMC article.
• Differentiation of the rat myelomonocytic leukemia cell line c-WRT-7 by in vitro culture with the rat bone marrow preadipocyte cell line REC A16.
  Tanaka T, Okamura S, Yasumoto S, Takeichi N, Kobayashi H, Niho Y. Tanaka T, et al. J Cancer Res Clin Oncol. 1993;119(6):335-41. doi: 10.1007/BF01208841. J Cancer Res Clin Oncol. 1993. PMID: 8449970

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley