A detailed mutational analysis of the eucaryotic tRNAmet1 gene promoter - PubMed (original) (raw)
A detailed mutational analysis of the eucaryotic tRNAmet1 gene promoter
W R Folk et al. Cell. 1983 Jun.
Abstract
We have isolated phage M13 clones containing the X. laevis trnamet1 gene, each having one or a few C leads to T transitions in the tRNA coding sequence. Nearly every G-C and C-G base pair in the tDNA has been mutagenized. The importance of these altered nucleotides in transcription by RNA polymerase III has been assessed by injecting the cloned DNAs into frog oocyte nuclei together with alpha-32P-GTP and measuring the synthesis of labeled tRNAmet1. Several G-C and C-G base pairs in the structural gene appear to be major promoter determinants, because when mutated, transcription is reduced 3-fold to 20-fold. Most of these determinants occur between nucleotides 7 to 19 and 49 to 61 in sequence regions highly conserved among eucaryotic as well as procaryotic tRNAs. Several additional G-C and C-G base pairs between these two regions also contribute to promoter activity; their location suggests that a stem-loop structure in the DNA encoding the tRNA's anticodon arm is important for RNA polymerase III promoter function.
Similar articles
- Mutations in Box B of the promoter of a eucaryotic tRNAPro gene affect rate of transcription, processing, and stability of the transcripts.
Traboni C, Ciliberto G, Cortese R. Traboni C, et al. Cell. 1984 Jan;36(1):179-87. doi: 10.1016/0092-8674(84)90087-4. Cell. 1984. PMID: 6559106 - Synthesis and maturation of Xenopus laevis methionine tRNA gene transcripts in homologous cell-free extracts.
Koski RA, Clarkson SG. Koski RA, et al. J Biol Chem. 1982 Apr 25;257(8):4514-21. J Biol Chem. 1982. PMID: 6802833 - A split promoter for a eucaryotic tRNA gene.
Hofstetter H, Kressman A, Birnstiel ML. Hofstetter H, et al. Cell. 1981 May;24(2):573-85. doi: 10.1016/0092-8674(81)90348-2. Cell. 1981. PMID: 7237560 - The functional significance of leader and trailer sequences in eukaryotic mRNAs.
Baralle FE. Baralle FE. Int Rev Cytol. 1983;81:71-106. doi: 10.1016/s0074-7696(08)62335-9. Int Rev Cytol. 1983. PMID: 6135669 Review. No abstract available. - Some aspects of double-stranded hairpin structures in heterogeneous nuclear RNA.
Naora H. Naora H. Int Rev Cytol. 1979;56:255-313. doi: 10.1016/s0074-7696(08)61824-0. Int Rev Cytol. 1979. PMID: 378887 Review. No abstract available.
Cited by
- Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes.
Sharp S, Dingermann T, Schaack J, Sharp JA, Burke DJ, DeRobertis EM, Söll D. Sharp S, et al. Nucleic Acids Res. 1983 Dec 20;11(24):8677-90. doi: 10.1093/nar/11.24.8677. Nucleic Acids Res. 1983. PMID: 6561520 Free PMC article. - Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor.
Willis I, Hottinger H, Pearson D, Chisholm V, Leupold U, Söll D. Willis I, et al. EMBO J. 1984 Jul;3(7):1573-80. doi: 10.1002/j.1460-2075.1984.tb02013.x. EMBO J. 1984. PMID: 6430697 Free PMC article. - Individual products of the adenovirus 12S and 13S EIa mRNAs stimulate viral EIIa and EIII expression at the transcriptional level.
Leff T, Elkaim R, Goding CR, Jalinot P, Sassone-Corsi P, Perricaudet M, Kédinger C, Chambon P. Leff T, et al. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4381-5. doi: 10.1073/pnas.81.14.4381. Proc Natl Acad Sci U S A. 1984. PMID: 6336330 Free PMC article. - In vitro mutagenesis and transcriptional analysis of a mouse ribosomal promoter element.
Skinner JA, Ohrlein A, Grummt I. Skinner JA, et al. Proc Natl Acad Sci U S A. 1984 Apr;81(7):2137-41. doi: 10.1073/pnas.81.7.2137. Proc Natl Acad Sci U S A. 1984. PMID: 6326106 Free PMC article. - A functional component of the sea urchin H2A gene modulator contains an extended sequence homology to a viral enhancer.
Grosschedl R, Mächler M, Rohrer U, Birnstiel ML. Grosschedl R, et al. Nucleic Acids Res. 1983 Dec 10;11(23):8123-36. doi: 10.1093/nar/11.23.8123. Nucleic Acids Res. 1983. PMID: 6324073 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous