Evidence for post-translational incorporation of a product of mevalonic acid into Swiss 3T3 cell proteins - PubMed (original) (raw)

. 1984 Aug 25;259(16):10175-80.

PMID: 6565705

Free article

Evidence for post-translational incorporation of a product of mevalonic acid into Swiss 3T3 cell proteins

R A Schmidt et al. J Biol Chem. 1984.

Free article

Abstract

Previous studies have identified several cellular requirements for mevalonic acid that appear unrelated to cholesterol, dolichol, or ubiquinone. To search for other products of mevalonic acid that might account for these requirements we cultured Swiss 3T3 cells in the presence of mevinolin, an inhibitor of mevalonic acid biosynthesis, then labeled the cells with exogenous radioactive mevalonic acid. Upon analyzing the radioactive material formed, we found that 40-50% of it was not extractable into lipid solvents, and that most of the lipid-insoluble material behaved like protein when treated with sodium dodecyl sulfate:chloroform:phenol, RNase, or proteinase K. Further analysis by electrophoresis revealed that radioactivity was associated with a few specific proteins that had apparent molecular weights of 13,000-58,000. Control experiments indicated that authentic radioactive (R)-mevalonic acid was the active precursor. Other lines of evidence suggested that mevalonate was first converted to an isoprenoid compound, then covalently incorporated into proteins by way of a cycloheximide-insensitive mechanism. These results suggest that Swiss 3T3 cells possess novel metabolic products of mevalonic acid metabolism that are formed by post-translational incorporation of isoprenoids into specific cell proteins.

PubMed Disclaimer

Cited by

Acylated proteins in Acholeplasma laidlawii.
Dahl CE, Sacktor NC, Dahl JS. Dahl CE, et al. J Bacteriol. 1985 Apr;162(1):445-7. doi: 10.1128/jb.162.1.445-447.1985. J Bacteriol. 1985. PMID: 3980443 Free PMC article.
The pseudorabies virus Us2 protein, a virion tegument component, is prenylated in infected cells.
Clase AC, Lyman MG, del Rio T, Randall JA, Calton CM, Enquist LW, Banfield BW. Clase AC, et al. J Virol. 2003 Nov;77(22):12285-98. doi: 10.1128/jvi.77.22.12285-12298.2003. J Virol. 2003. PMID: 14581565 Free PMC article.
GTP-binding mutants of rab1 and rab2 are potent inhibitors of vesicular transport from the endoplasmic reticulum to the Golgi complex.
Tisdale EJ, Bourne JR, Khosravi-Far R, Der CJ, Balch WE. Tisdale EJ, et al. J Cell Biol. 1992 Nov;119(4):749-61. doi: 10.1083/jcb.119.4.749. J Cell Biol. 1992. PMID: 1429835 Free PMC article.
RhoB prenylation is driven by the three carboxyl-terminal amino acids of the protein: evidenced in vivo by an anti-farnesyl cysteine antibody.
Baron R, Fourcade E, Lajoie-Mazenc I, Allal C, Couderc B, Barbaras R, Favre G, Faye JC, Pradines A. Baron R, et al. Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11626-31. doi: 10.1073/pnas.97.21.11626. Proc Natl Acad Sci U S A. 2000. PMID: 11027361 Free PMC article.
v-K-ras leads to preferential farnesylation of p21(ras) in FRTL-5 cells: multiple interference with the isoprenoid pathway.
Laezza C, Di Marzo V, Bifulco M. Laezza C, et al. Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13646-51. doi: 10.1073/pnas.95.23.13646. Proc Natl Acad Sci U S A. 1998. PMID: 9811854 Free PMC article.

Evidence for post-translational incorporation of a product of mevalonic acid into Swiss 3T3 cell proteins - PubMed (original) (raw)