Production and characterization of a monoclonal antibody directed against the 43,000-dalton v1 polypeptide from Torpedo marmorata electric organ - PubMed (original) (raw)
Production and characterization of a monoclonal antibody directed against the 43,000-dalton v1 polypeptide from Torpedo marmorata electric organ
H O Nghiêm et al. Proc Natl Acad Sci U S A. 1983 Oct.
Abstract
Subsynaptic membrane fragments prepared from Torpedo marmorata electric organ contain, in addition to the acetylcholine receptor polypeptides, a major protein band of apparent molecular mass 43,000 daltons. On two-dimensional gels, this band yields three spots referred to as v1, v2, and v3. Monoclonal antibodies against the 43,000-dalton proteins were developed in CBA mice. One of them reacted exclusively with the v1 polypeptide but not with v2 and v3. Staining by the "immunogold" reaction followed by observation by electron microscopy showed that this antibody exclusively labeled the innervated membrane of T. marmorata electroplaque on its cytoplasmic face. Electroblots of one-dimensional gels of membrane preparations from 80-mm embryo electric organ were prepared. After reaction with the anti-v1 monoclonal antibody, a strongly stained 43,000-dalton band was revealed.
Similar articles
- Peripheral proteins of postsynaptic membranes from Torpedo electric organ identified with monoclonal antibodies.
Froehner SC. Froehner SC. J Cell Biol. 1984 Jul;99(1 Pt 1):88-96. doi: 10.1083/jcb.99.1.88. J Cell Biol. 1984. PMID: 6376523 Free PMC article. - Monoclonal antibody directed against the 43 000 dalton v1 polypeptide from Torpedo marmorata electric organ.
Nghiêm HO, Cartaud J, Dubreuil C, Kordeli C, Petit-Koskas E, Buttin G, Changeux JP. Nghiêm HO, et al. Dev Biol Stand. 1984;57:393-8. Dev Biol Stand. 1984. PMID: 6526147 - Cytoskeletal proteins at the cholinergic synapse: distribution of desmin, actin, fodrin, neurofilaments, and tubulin in Torpedo electric organ.
Walker JH, Boustead CM, Witzemann V, Shaw G, Weber K, Osborn M. Walker JH, et al. Eur J Cell Biol. 1985 Jul;38(1):123-33. Eur J Cell Biol. 1985. PMID: 3896807 - nu 1, a Mr 43,000 component of postsynaptic membranes, is a protein kinase.
Gordon AS, Milfay D. Gordon AS, et al. Proc Natl Acad Sci U S A. 1986 Jun;83(12):4172-4. doi: 10.1073/pnas.83.12.4172. Proc Natl Acad Sci U S A. 1986. PMID: 3459169 Free PMC article. - Acetylcholine receptor-enriched membranes from Torpedo marmorata electroplax, prepared by liquid partitioning.
Heilbronn E, Björk C, Elfman L, Hartman A, Mattsson C. Heilbronn E, et al. Adv Cytopharmacol. 1979;3:151-8. Adv Cytopharmacol. 1979. PMID: 382780 Review. No abstract available.
Cited by
- Association of acetylcholine receptors with peripheral membrane proteins: evidence from antibody-induced coaggregation.
Bloch RJ, Sealock R, Pumplin DW, Luther PW, Froehner SC. Bloch RJ, et al. J Membr Biol. 1994 Feb;138(1):13-28. doi: 10.1007/BF00211065. J Membr Biol. 1994. PMID: 8189428 - Ultrastructural localization of the Mr 43,000 protein and the acetylcholine receptor in Torpedo postsynaptic membranes using monoclonal antibodies.
Sealock R, Wray BE, Froehner SC. Sealock R, et al. J Cell Biol. 1984 Jun;98(6):2239-44. doi: 10.1083/jcb.98.6.2239. J Cell Biol. 1984. PMID: 6725413 Free PMC article. - The 43-K protein, v1, associated with acetylcholine receptor containing membrane fragments is an actin-binding protein.
Walker JH, Boustead CM, Witzemann V. Walker JH, et al. EMBO J. 1984 Oct;3(10):2287-90. doi: 10.1002/j.1460-2075.1984.tb02127.x. EMBO J. 1984. PMID: 6389118 Free PMC article. - Peripheral proteins of postsynaptic membranes from Torpedo electric organ identified with monoclonal antibodies.
Froehner SC. Froehner SC. J Cell Biol. 1984 Jul;99(1 Pt 1):88-96. doi: 10.1083/jcb.99.1.88. J Cell Biol. 1984. PMID: 6376523 Free PMC article. - Complete nucleotide sequence of Torpedo marmorata mRNA coding for the 43,000-dalton nu 2 protein: muscle-specific creatine kinase.
Giraudat J, Devillers-Thiery A, Perriard JC, Changeux JP. Giraudat J, et al. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7313-7. doi: 10.1073/pnas.81.23.7313. Proc Natl Acad Sci U S A. 1984. PMID: 6095285 Free PMC article.
References
- Nature. 1970 Aug 15;227(5259):680-5 - PubMed
- J Biol Chem. 1975 May 25;250(10):4007-21 - PubMed
- Nature. 1975 Aug 7;256(5517):495-7 - PubMed
- Eur J Biochem. 1977 Oct 17;80(1):215-24 - PubMed
- J Cell Sci. 1978 Feb;29:313-37 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources