Interleukin 2 dependence of human natural killer (NK) cell activity - PubMed (original) (raw)
. 1983 Apr;130(4):1970-3.
- PMID: 6601144
Interleukin 2 dependence of human natural killer (NK) cell activity
W Domzig et al. J Immunol. 1983 Apr.
Abstract
When purified populations of human natural killer (NK) cells were tested for cytotoxic activity in the presence of partially purified preparations of human interleukin 2 (IL 2), a definite, dose-dependent linear increase in reactivity was observed. To determine whether such augmentation by IL 2 might reflect an important aspect of the physiologic regulation of NK activity, we examined the effects of monoclonal antibodies against human IL 2 on spontaneous NK activity. The presence of such antibodies during the 4-hr cytotoxicity assay resulted in significant inhibition of NK activity, and when the NK cells were pretreated for 16 to 20 hr with anti-IL 2, little or no activity remained. These data suggest that the spontaneous cytotoxic activity of NK cells is dependent on their continued exposure to IL 2. The reduction in NK activity resulting from treatment with anti-IL 2 could be at least partially restored by exposure to only low amounts of partially purified IL 2. These data have provided the basis for formulating a novel model of NK cell activation.
Similar articles
- Effects of natural and recombinant IL 2 on regulation of IFN gamma production and natural killer activity: lack of involvement of the Tac antigen for these immunoregulatory effects.
Ortaldo JR, Mason AT, Gerard JP, Henderson LE, Farrar W, Hopkins RF 3rd, Herberman RB, Rabin H. Ortaldo JR, et al. J Immunol. 1984 Aug;133(2):779-83. J Immunol. 1984. PMID: 6203980 - Interleukin-2-induced activation of natural killer activity in spleen cells from old and young mice.
Saxena RK, Saxena QB, Adler WH. Saxena RK, et al. Immunology. 1984 Apr;51(4):719-26. Immunology. 1984. PMID: 6608487 Free PMC article. - Dual effects of cytokines in regulation of MHC-unrestricted cell mediated cytotoxicity.
Reiter Z. Reiter Z. Crit Rev Immunol. 1993;13(1):1-34. Crit Rev Immunol. 1993. PMID: 8466640 Review. - Natural killer cells.
Herberman RB. Herberman RB. Annu Rev Med. 1986;37:347-52. doi: 10.1146/annurev.me.37.020186.002023. Annu Rev Med. 1986. PMID: 3518610 Review.
Cited by
- Interleukin 2 enhances natural killing of varicella-zoster virus-infected targets.
Ito M, Bandyopadhyay S, Matsumoto-Kobayashi M, Clark SC, Miller D, Starr SE. Ito M, et al. Clin Exp Immunol. 1986 Jul;65(1):182-9. Clin Exp Immunol. 1986. PMID: 3098472 Free PMC article. - Functional consequences of interleukin 2 receptor expression on resting human lymphocytes. Identification of a novel natural killer cell subset with high affinity receptors.
Caligiuri MA, Zmuidzinas A, Manley TJ, Levine H, Smith KA, Ritz J. Caligiuri MA, et al. J Exp Med. 1990 May 1;171(5):1509-26. doi: 10.1084/jem.171.5.1509. J Exp Med. 1990. PMID: 1692080 Free PMC article. - Natural killer cell activity in Sjögren's syndrome and systemic lupus erythematosus: stimulation with interferons and interleukin-2 and correlation with immune complexes.
Struyf NJ, Snoeck HW, Bridts CH, De Clerck LS, Stevens WJ. Struyf NJ, et al. Ann Rheum Dis. 1990 Sep;49(9):690-3. doi: 10.1136/ard.49.9.690. Ann Rheum Dis. 1990. PMID: 1700674 Free PMC article. - Natural killer cell activity during measles.
Griffin DE, Ward BJ, Jauregui E, Johnson RT, Vaisberg A. Griffin DE, et al. Clin Exp Immunol. 1990 Aug;81(2):218-24. doi: 10.1111/j.1365-2249.1990.tb03321.x. Clin Exp Immunol. 1990. PMID: 1696863 Free PMC article. - Human natural killer cells do not inhibit growth of Cryptococcus neoformans in the absence of antibody.
Miller MF, Mitchell TG, Storkus WJ, Dawson JR. Miller MF, et al. Infect Immun. 1990 Mar;58(3):639-45. doi: 10.1128/iai.58.3.639-645.1990. Infect Immun. 1990. PMID: 2407651 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources