Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components - PubMed (original) (raw)

Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components

M J Lohka et al. Science. 1983.

Abstract

A cell-free preparation of the cytoplasm from activated eggs of Rana pipiens induces, in demembranated sperm nuclei of Xenopus laevis, formation of a nuclear envelope, chromatin decondensation, initiation of DNA synthesis, and chromosome condensation. Both soluble and particulate cytoplasmic constituents are required to initiate these processes in vitro. The observed changes resemble processes occurring during fertilization and the mitotic cycle in early amphibian embryos. Therefore, this cell-free system may be useful in biochemical analysis of the interactions of nucleus and cytoplasm that control nuclear behavior.

PubMed Disclaimer

Similar articles

• Roles of cytosol and cytoplasmic particles in nuclear envelope assembly and sperm pronuclear formation in cell-free preparations from amphibian eggs.
  Lohka MJ, Masui Y. Lohka MJ, et al. J Cell Biol. 1984 Apr;98(4):1222-30. doi: 10.1083/jcb.98.4.1222. J Cell Biol. 1984. PMID: 6609160 Free PMC article.
• Development of pronuclei from human spermatozoa injected microsurgically into frog (Xenopus) eggs.
  Ohsumi K, Katagiri C, Yanagimachi R. Ohsumi K, et al. J Exp Zool. 1986 Mar;237(3):319-25. doi: 10.1002/jez.1402370304. J Exp Zool. 1986. PMID: 3701290
• Remodeling the sperm nucleus into a male pronucleus at fertilization.
  Collas P, Poccia D. Collas P, et al. Theriogenology. 1998 Jan 1;49(1):67-81. doi: 10.1016/s0093-691x(97)00403-2. Theriogenology. 1998. PMID: 10732122 Review.
• Chromatin remodeling in mammalian zygotes.
  Perreault SD. Perreault SD. Mutat Res. 1992 Dec;296(1-2):43-55. doi: 10.1016/0165-1110(92)90031-4. Mutat Res. 1992. PMID: 1279407 Review.

Cited by

• Optimized expansion microscopy reveals species-specific spindle microtubule organization in Xenopus egg extracts.
  Guilloux G, Kitaoka M, Mocaer K, Heichette C, Duchesne L, Heald R, Pecot T, Gibeaux R. Guilloux G, et al. bioRxiv [Preprint]. 2024 Sep 25:2024.09.11.612005. doi: 10.1101/2024.09.11.612005. bioRxiv. 2024. PMID: 39314487 Free PMC article. Preprint.
• Protocol for the purification of replisomes from the Xenopus laevis egg extract system for single-particle cryo-EM analysis.
  Passaretti P, Cvetkovic MA, Costa A, Gambus A. Passaretti P, et al. STAR Protoc. 2024 Sep 20;5(3):103237. doi: 10.1016/j.xpro.2024.103237. Epub 2024 Aug 8. STAR Protoc. 2024. PMID: 39126657 Free PMC article.
• Xenopus cell-free extracts and their applications in cell biology study.
  Liu J, Zhang C. Liu J, et al. Biophys Rep. 2023 Aug 31;9(4):195-205. doi: 10.52601/bpr.2023.230016. Biophys Rep. 2023. PMID: 38516620 Free PMC article.
• What are the contributions of maternal and paternal traits to fecundity and offspring development? A case study in an amphibian species, the spined toad Bufo spinosus.
  Renoirt M, Angelier F, Cheron M, Brischoux F. Renoirt M, et al. Curr Zool. 2022 Sep 8;69(5):527-534. doi: 10.1093/cz/zoac072. eCollection 2023 Oct. Curr Zool. 2022. PMID: 37637310 Free PMC article.
• Dependence of removal of sperm-specific proteins from Xenopus sperm nuclei on the phosphorylation state of nucleoplasmin.
  Ohsumi K, Shimada A, Okumura E, Kishimoto T, Katagiri C. Ohsumi K, et al. Dev Growth Differ. 1995 Jun;37(3):329-336. doi: 10.1046/j.1440-169X.1995.t01-1-00011.x. Dev Growth Differ. 1995. PMID: 37281801

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon

• Other Literature Sources

  • The Lens - Patent Citations