Protein loss during nuclear isolation - PubMed (original) (raw)

Protein loss during nuclear isolation

P L Paine et al. J Cell Biol. 1983 Oct.

Abstract

Cryomicrodissection makes possible the measurement of the entire in vivo protein content of the amphibian oocyte nucleus and provides a heretofore missing baseline for estimating protein loss during nuclear isolation by other methods. When oocyte nuclei are isolated into an aqueous medium, they lose 95% of their protein with a half-time of 250 s. This result implies an even more rapid loss of protein from aqueously isolated nuclei of ordinary-size cells.

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