Human actin genes are single copy for alpha-skeletal and alpha-cardiac actin but multicopy for beta- and gamma-cytoskeletal genes: 3' untranslated regions are isotype specific but are conserved in evolution - PubMed (original) (raw)
Human actin genes are single copy for alpha-skeletal and alpha-cardiac actin but multicopy for beta- and gamma-cytoskeletal genes: 3' untranslated regions are isotype specific but are conserved in evolution
P Ponte et al. Mol Cell Biol. 1983 Oct.
Abstract
We have constructed isotype-specific subclones from the 3' untranslated regions of alpha-skeletal, alpha-cardiac, beta-cytoskeletal, and gamma-cytoskeletal actin cDNAs. These clones have been used as hybridization probes to assay the number and organization of these actin isotypes in the human genome. Hybridization of these probes to human genomic actin clones (Engel et al., Proc. Natl. Acad. Sci. U.S.A. 78:4674-4678, 1981; Engel et al., Mol. Cell. Biol. 2:674-684, 1982) has allowed the unambiguous assignment of the genomic clones to isotypically defined actin subfamilies. In addition, only one isotype-specific probe hybridizes to each actin-containing gene, with a single exception. This result suggests that the multiple actin genes in the human genome are not closely linked. Genomic DNA blots probed with these subclones under stringent conditions demonstrate that the alpha-skeletal and alpha-cardiac muscle actin genes are single copy, whereas the cytoskeletal actins, beta and gamma, are present in multiple copies in the human genome. Most of the actin genes of other mammals are cytoplasmic as well. These observations have important implications for the evolution of multigene families.
Similar articles
- alpha-skeletal and alpha-cardiac actin genes are coexpressed in adult human skeletal muscle and heart.
Gunning P, Ponte P, Blau H, Kedes L. Gunning P, et al. Mol Cell Biol. 1983 Nov;3(11):1985-95. doi: 10.1128/mcb.3.11.1985-1995.1983. Mol Cell Biol. 1983. PMID: 6689196 Free PMC article. - Nucleotide sequence of the human gamma cytoskeletal actin mRNA: anomalous evolution of vertebrate non-muscle actin genes.
Erba HP, Gunning P, Kedes L. Erba HP, et al. Nucleic Acids Res. 1986 Jul 11;14(13):5275-94. doi: 10.1093/nar/14.13.5275. Nucleic Acids Res. 1986. PMID: 3737401 Free PMC article. - Isolation and characterization of six different chicken actin genes.
Chang KS, Zimmer WE Jr, Bergsma DJ, Dodgson JB, Schwartz RJ. Chang KS, et al. Mol Cell Biol. 1984 Nov;4(11):2498-508. doi: 10.1128/mcb.4.11.2498-2508.1984. Mol Cell Biol. 1984. PMID: 6513927 Free PMC article. - Cardiac actin is the major actin gene product in skeletal muscle cell differentiation in vitro.
Bains W, Ponte P, Blau H, Kedes L. Bains W, et al. Mol Cell Biol. 1984 Aug;4(8):1449-53. doi: 10.1128/mcb.4.8.1449-1453.1984. Mol Cell Biol. 1984. PMID: 6493226 Free PMC article.
Cited by
- Insights on Droplet Digital PCR-Based Cellular Kinetics and Biodistribution Assay Support for CAR-T Cell Therapy.
Sugimoto H, Chen S, Minembe JP, Chouitar J, He X, Wang H, Fang X, Qian MG. Sugimoto H, et al. AAPS J. 2021 Mar 2;23(2):36. doi: 10.1208/s12248-021-00560-6. AAPS J. 2021. PMID: 33655393 Free PMC article. - Accurate measurement of mitochondrial DNA deletion level and copy number differences in human skeletal muscle.
Grady JP, Murphy JL, Blakely EL, Haller RG, Taylor RW, Turnbull DM, Tuppen HA. Grady JP, et al. PLoS One. 2014 Dec 4;9(12):e114462. doi: 10.1371/journal.pone.0114462. eCollection 2014. PLoS One. 2014. PMID: 25474153 Free PMC article. - Determination of copy number and linkage relationships among five actin gene subfamilies in Petunia hybrida.
McLean M, Baird WV, Gerats AG, Meagher RB. McLean M, et al. Plant Mol Biol. 1988 Sep;11(5):663-72. doi: 10.1007/BF00017466. Plant Mol Biol. 1988. PMID: 24272500 - Beta-Actin Is Involved in Modulating Erythropoiesis during Development by Fine-Tuning Gata2 Expression Levels.
Tondeleir D, Drogat B, Slowicka K, Bakkali K, Bartunkova S, Goossens S, Haigh JJ, Ampe C. Tondeleir D, et al. PLoS One. 2013 Jun 26;8(6):e67855. doi: 10.1371/journal.pone.0067855. Print 2013. PLoS One. 2013. PMID: 23840778 Free PMC article. - Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists.
Kandasamy MK, McKinney EC, Roy E, Meagher RB. Kandasamy MK, et al. Plant Cell. 2012 May;24(5):2041-57. doi: 10.1105/tpc.111.095281. Epub 2012 May 15. Plant Cell. 2012. PMID: 22589468 Free PMC article.
References
- Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 - PubMed
- Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 - PubMed
- Proc Natl Acad Sci U S A. 1978 Mar;75(3):1106-10 - PubMed
- FEBS Lett. 1979 Jun 15;102(2):219-22 - PubMed
- Differentiation. 1979;14(3):123-33 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources