Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis - PubMed (original) (raw)
Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis
C J Barnstable et al. Cell. 1978 May.
Abstract
Antibody-secreting hybrid cells have been derived from a fusion between mouse myeloma cells and spleen cells from a mouse immunized with membrane from human tonsil lymphocyte preparations. Hybrids secreting antibodies to cell surface antigens were detected by assaying culture supernatants for antibody binding to human tonsil cells. Six different antibodies (called W6/1, /28, /32, /34, /45 and /46 were analyzed. These were either against antigens of wide tissue distribution (W6/32, /34, and /46) or mainly on erythrocytes (W6/1 and W6/28). One of the anti-erythrocyte antibodies (W6/1) detected a polymorphic antigen, since blood group A1 and A2 erythrocytes were labeled while B and O were not. Antibodies W6/34, /45 and /46 were all against antigens which were mapped to the short arm of chromosome 11 by segregation analysis of mouse-human hybrids. Immunoprecipitation studies suggest that W6/45 antigen may be a protein of 16,000 dalton, apparent molecular weight, while W6/34 and /46 antigens could not be detected by this technique. Antibody W6/32 is against a determinant common to most, if not all, of the 43,000 dalton molecular weight chains of HLA-A, B and C antigens. This was established by somatic cell genetic techniques and by immunoprecipitation analysis. Tonsil leucocytes bound 370,000 W6/32 antibody molecules per cell at saturation. The hybrid myelomas W6/32 and W6/34 have been cloned, and both secrete an IgG2 antibody. W6/32 cells were grown in mice, and the serum of the tumor-bearing animals contained greater than 10 mg/ml of monoclonal antibody. The experiments established the usefulness of the bybrid myeloma technique in preparing monospecific antibodies against human cell surface antigens. In particular, this study highlights the possibilities not only of obtaining reagents for somatic cell genetics, but also of obtaining mouse antibodies detecting human antigenic polymorphisms.
Similar articles
- Recognition of interspecies hybrid class I histocompatibility antigens by antigen-specific cytolytic T lymphocytes.
Maziarz R, Allen H, Strominger JL, Flavell R, Biro PA, Burakoff SJ. Maziarz R, et al. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6276-80. doi: 10.1073/pnas.82.18.6276. Proc Natl Acad Sci U S A. 1985. PMID: 3875858 Free PMC article. - A monoclonal anti-HLA antibody recognizes a mouse tumor-associated antigen.
Evan GI, Lennox ES, Alderson T, Croft L. Evan GI, et al. Eur J Immunol. 1983 Feb;13(2):160-6. doi: 10.1002/eji.1830130213. Eur J Immunol. 1983. PMID: 6601010 - [Preliminary serological analysis for identification of direct monoclonal antibodies against surface structures of HLA typed human lymphocytes].
Richiardi P, Crepaldi T, Amoroso A. Richiardi P, et al. G Batteriol Virol Immunol. 1980 Jul-Dec;73(7-12):165-79. G Batteriol Virol Immunol. 1980. PMID: 7030845 Review. Italian. - Production of monoclonal antibodies to bovine leukocyte cell-surface components.
Nickerson SC, Shapiro RP, Guidry AJ, Srikumaran S, Goldsby RA. Nickerson SC, et al. J Dairy Sci. 1983 Jul;66(7):1547-58. doi: 10.3168/jds.S0022-0302(83)81971-7. J Dairy Sci. 1983. PMID: 6350394 Review.
Cited by
- Conformational Alterations of the Cell Surface of Monomeric and Dimeric β2m-Free HLA-I (Proto-HLA) May Enable Novel Immune Functions in Health and Disease.
Ravindranath MH, Ravindranath NM, Amato-Menker CJ, Hilali FE, Filippone EJ. Ravindranath MH, et al. Curr Issues Mol Biol. 2024 Jul 4;46(7):6961-6985. doi: 10.3390/cimb46070416. Curr Issues Mol Biol. 2024. PMID: 39057057 Free PMC article. Review. - A monoclonal antibody that recognizes a unique 13-residue epitope in the cytoplasmic tail of HLA-E.
Verhaar ER, Gan J, Buhl S, Li Z, Horowitz A, Ploegh HL. Verhaar ER, et al. Mol Immunol. 2024 Aug;172:56-67. doi: 10.1016/j.molimm.2024.06.004. Epub 2024 Jun 19. Mol Immunol. 2024. PMID: 38901180 - Dual role of the peptide-loading complex as proofreader and limiter of MHC-I presentation.
Brunnberg J, Barends M, Frühschulz S, Winter C, Battin C, de Wet B, Cole DK, Steinberger P, Tampé R. Brunnberg J, et al. Proc Natl Acad Sci U S A. 2024 May 28;121(22):e2321600121. doi: 10.1073/pnas.2321600121. Epub 2024 May 21. Proc Natl Acad Sci U S A. 2024. PMID: 38771881 Free PMC article. - TOFIMS mass spectrometry-based immunopeptidomics refines tumor antigen identification.
Hoenisch Gravel N, Nelde A, Bauer J, Mühlenbruch L, Schroeder SM, Neidert MC, Scheid J, Lemke S, Dubbelaar ML, Wacker M, Dengler A, Klein R, Mauz PS, Löwenheim H, Hauri-Hohl M, Martin R, Hennenlotter J, Stenzl A, Heitmann JS, Salih HR, Rammensee HG, Walz JS. Hoenisch Gravel N, et al. Nat Commun. 2023 Nov 17;14(1):7472. doi: 10.1038/s41467-023-42692-7. Nat Commun. 2023. PMID: 37978195 Free PMC article. - Mass Spectrometric Profiling of HLA-B44 Peptidomes Provides Evidence for Tapasin-Mediated Tryptophan Editing.
Kaur A, Surnilla A, Zaitouna AJ, Mumphrey MB, Basrur V, Grigorova I, Cieslik M, Carrington M, Nesvizhskii AI, Raghavan M. Kaur A, et al. J Immunol. 2023 Nov 1;211(9):1298-1307. doi: 10.4049/jimmunol.2300232. J Immunol. 2023. PMID: 37737643 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous