A platelet membrane protein expressed during platelet activation and secretion. Studies using a monoclonal antibody specific for thrombin-activated platelets - PubMed (original) (raw)
. 1984 Jul 25;259(14):9121-6.
- PMID: 6746643
Free article
A platelet membrane protein expressed during platelet activation and secretion. Studies using a monoclonal antibody specific for thrombin-activated platelets
S Hsu-Lin et al. J Biol Chem. 1984.
Free article
Abstract
To identify structures on the platelet surface which become expressed after platelet activation, we have prepared murine monoclonal antibodies specific for thrombin-activated platelets. Hybridomas were screened for clones producing antibodies which bound to thrombin-activated platelets but not to resting platelets. Clone KC4 was identified. The binding of purified I-labeled KC4 antibody, an IgG1k, to thrombin-activated platelets was saturable. Minimal binding was observed to resting platelets. The interaction of antibody with thrombin-activated platelets was characterized by a binding constant, KD, of 7.2 +/- 0.4 nM and revealed 13,400 +/- 3,000 binding sites per platelet. The presence of Ca2+ or EDTA, a pH ranging from 4 to 10, or high ionic strength had no influence on antigen-antibody interaction. The KC4 antigen was expressed on the platelet surface after activation with ADP, collagen, epinephrine, or thrombin. The extent of [14C] serotonin release during activation was directly proportional to the availability of antigen on the platelet surface regardless of agonist or platelet aggregation. The antibody is directed against a single protein which migrated between GPIIb and GPIIa after sodium dodecyl sulfate gel electrophoresis. This protein was purified from platelet membranes by immunoaffinity chromatography using KC4 antibody-agarose and demonstrated an apparent molecular weight of 140,000 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis under both nonreducing and reducing conditions. Of the cells examined, only platelets contained this protein. These results indicate that platelet secretion is associated with the expression of an Mr = 140,000 integral membrane protein composed of a single polypeptide chain. This protein may be component of the internal granule membrane which is fused with the plasma membrane during activation.
Similar articles
- A monoclonal antibody to a membrane glycoprotein binds only to activated platelets.
McEver RP, Martin MN. McEver RP, et al. J Biol Chem. 1984 Aug 10;259(15):9799-804. J Biol Chem. 1984. PMID: 6746667 - Human platelet osteonectin: release, surface expression, and partial characterization.
Kelm RJ Jr, Mann KG. Kelm RJ Jr, et al. Blood. 1990 Mar 1;75(5):1105-13. Blood. 1990. PMID: 2306517 - Membrane changes associated with platelet activation. Exposure of actin on the platelet surface after thrombin-induced secretion.
George JN, Lyons RM, Morgan RK. George JN, et al. J Clin Invest. 1980 Jul;66(1):1-9. doi: 10.1172/JCI109821. J Clin Invest. 1980. PMID: 6772667 Free PMC article. - All platelets are not equal: COAT platelets.
Alberio LJ, Clemetson KJ. Alberio LJ, et al. Curr Hematol Rep. 2004 Sep;3(5):338-43. Curr Hematol Rep. 2004. PMID: 15341700 Review.
Cited by
- Cold temperature induces a TRPM8-independent calcium release from the endoplasmic reticulum in human platelets.
Stratiievska A, Filippova O, Özpolat T, Byrne D, Bailey SL, Chauhan A, Mollica MY, Harris J, Esancy K, Chen J, Dhaka AK, Sniadecki NJ, López JA, Stolla M. Stratiievska A, et al. PLoS One. 2024 Mar 4;19(3):e0289395. doi: 10.1371/journal.pone.0289395. eCollection 2024. PLoS One. 2024. PMID: 38437228 Free PMC article. - Cold temperature induces a TRPM8-independent calcium release from the endoplasmic reticulum in human platelets.
Stratiievska A, Filippova O, Özpolat T, Byrne D, Bailey SL, Mollica MY, Harris J, Esancy K, Chen J, Dhaka AK, Sniadecki NJ, López JA, Stolla M. Stratiievska A, et al. bioRxiv [Preprint]. 2023 Jul 19:2023.07.19.549670. doi: 10.1101/2023.07.19.549670. bioRxiv. 2023. PMID: 37502986 Free PMC article. Updated. Preprint. - Dematin Regulates Calcium Mobilization, Thrombosis, and Early Akt Activation in Platelets.
Fritz DI, Ding Y, Merrill-Skoloff G, Flaumenhaft R, Hanada T, Chishti AH. Fritz DI, et al. Mol Cell Biol. 2023;43(6):283-299. doi: 10.1080/10985549.2023.2210033. Epub 2023 May 22. Mol Cell Biol. 2023. PMID: 37216480 Free PMC article. - Inhalation of an RNA aptamer that selectively binds extracellular histones protects from acute lung injury.
Lei B, Wang C, Snow K, Graton ME, Tighe RM, Fager AM, Hoffman MR, Giangrande PH, Miller FJ Jr. Lei B, et al. Mol Ther Nucleic Acids. 2023 Feb 24;31:662-673. doi: 10.1016/j.omtn.2023.02.021. eCollection 2023 Mar 14. Mol Ther Nucleic Acids. 2023. PMID: 36910716 Free PMC article. - RGS10 and RGS18 differentially limit platelet activation, promote platelet production, and prolong platelet survival.
DeHelian D, Gupta S, Wu J, Thorsheim C, Estevez B, Cooper M, Litts K, Lee-Sundlov MM, Hoffmeister KM, Poncz M, Ma P, Brass LF. DeHelian D, et al. Blood. 2020 Oct 8;136(15):1773-1782. doi: 10.1182/blood.2019003251. Blood. 2020. PMID: 32542378 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous