Purification and partial characterization of a thiol proteinase from the thermophilic fungus Humicola lanuginosa - PubMed (original) (raw)
Purification and partial characterization of a thiol proteinase from the thermophilic fungus Humicola lanuginosa
S Shenolikar et al. Biochem J. 1982.
Abstract
An extracellular thiol proteinase was produced by the growth of a thermophilic fungus, Humicola lanuginosa, on a medium containing 2% casein, and was purified to virtual homogeneity by affinity chromatography on organomercurial columns. The essential thiol group for activity was confirmed by the inhibition of the enzyme by p-chloromercuribenzoate and mercuric ions. The enzyme, purified 27-fold from the extracellular fluid, exhibited an Mr of 23700 on gel filtration and sedimentation equilibrium. The H. lanuginosa proteinase preferentially cleaves at the C-terminal end of hydrophobic amino acid residues. This proteinase differed from the plant enzyme papain in its interaction with three affinity matrices and its substrate specificity towards synthetic substrates. This enzyme represents a unique example of a thiol proteinase obtained from a fungal source.
Similar articles
- Characterization of a chelator-resistant proteinase from Thermus strain Rt4A2.
Freeman SA, Peek K, Prescott M, Daniel R. Freeman SA, et al. Biochem J. 1993 Oct 15;295 ( Pt 2)(Pt 2):463-9. doi: 10.1042/bj2950463. Biochem J. 1993. PMID: 8240244 Free PMC article. - [Carboxylic proteinase from Trichoderma lignorum].
Rudenskaia GN, Osterman AL, Stepanov VM. Rudenskaia GN, et al. Biokhimiia. 1980 Apr;45(4):710-7. Biokhimiia. 1980. PMID: 6991003 Russian. - Purification and properties of xylanase from the thermophilic fungus, Humicola lanuginosa (Griffon and Maublanc) Bunce.
Anand L, Krishnamurthy S, Vithayathil PJ. Anand L, et al. Arch Biochem Biophys. 1990 Feb 1;276(2):546-53. doi: 10.1016/0003-9861(90)90757-p. Arch Biochem Biophys. 1990. PMID: 2306111 - Purification and characterization of an extracellular thiol-containing serine proteinase from Thermomyces lanuginosus.
Hasnain S, Adeli K, Storer AC. Hasnain S, et al. Biochem Cell Biol. 1992 Feb;70(2):117-22. doi: 10.1139/o92-017. Biochem Cell Biol. 1992. PMID: 1510823 - Purification and properties of a neutral thiol protease from larval trematode parasite Paragonimus westermani metacercariae.
Yamakami K, Hamajima F. Yamakami K, et al. Comp Biochem Physiol B. 1987;87(3):643-8. doi: 10.1016/0305-0491(87)90065-4. Comp Biochem Physiol B. 1987. PMID: 3304826
Cited by
- Thermophilic fungi: their physiology and enzymes.
Maheshwari R, Bharadwaj G, Bhat MK. Maheshwari R, et al. Microbiol Mol Biol Rev. 2000 Sep;64(3):461-88. doi: 10.1128/MMBR.64.3.461-488.2000. Microbiol Mol Biol Rev. 2000. PMID: 10974122 Free PMC article. Review. - Cloning, expression, and characterization of serine protease from thermophilic fungus Thermoascus aurantiacus var. levisporus.
Li AN, Xie C, Zhang J, Zhang J, Li DC. Li AN, et al. J Microbiol. 2011 Feb;49(1):121-9. doi: 10.1007/s12275-011-9355-6. Epub 2011 Mar 3. J Microbiol. 2011. PMID: 21369989 - A Comparative Study of New Aspergillus Strains for Proteolytic Enzymes Production by Solid State Fermentation.
Ortiz GE, Noseda DG, Ponce Mora MC, Recupero MN, Blasco M, Albertó E. Ortiz GE, et al. Enzyme Res. 2016;2016:3016149. doi: 10.1155/2016/3016149. Epub 2016 Feb 16. Enzyme Res. 2016. PMID: 26989505 Free PMC article. - Characterization of an exocellular serine-thiol proteinase activity in Paracoccidioides brasiliensis.
Carmona AK, Puccia R, Oliveira MC, Rodrigues EG, Juliano L, Travassos LR. Carmona AK, et al. Biochem J. 1995 Jul 1;309 ( Pt 1)(Pt 1):209-14. doi: 10.1042/bj3090209. Biochem J. 1995. PMID: 7619058 Free PMC article.
References
- Arch Biochem Biophys. 1968 Mar 20;124(1):70-8 - PubMed
- Nature. 1968 Jun 8;218(5145):929-32 - PubMed
- J Biol Chem. 1969 Aug 25;244(16):4406-12 - PubMed
- Biochim Biophys Acta. 1970 Mar 31;200(3):593-5 - PubMed
- J Biol Chem. 1970 Jun 10;245(11):2765-72 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources