Sensitivity of K1-encapsulated Escherichia coli to killing by the bactericidal/permeability-increasing protein of rabbit and human neutrophils - PubMed (original) (raw)

Comparative Study

Sensitivity of K1-encapsulated Escherichia coli to killing by the bactericidal/permeability-increasing protein of rabbit and human neutrophils

J Weiss et al. Infect Immun. 1982 Dec.

Abstract

The presence of K1 capsular polysaccharides increases the resistance of Escherichia coli to killing by serum and phagocytosis by polymorphonuclear leukocytes (PMNs). To determine whether K1 capsule impedes the action of intracellular bactericidal systems of PMNs, we compared the sensitivity of several K1-encapsulated and non-encapsulated strains of E. coli to killing by the bactericidal/permeability-increasing protein (BPI) isolated from rabbit and human PMNs. BPI appears to be the principal bactericidal agent of PMNs toward E. coli and other gram-negative bacteria (Weiss et al., J. Clin. Invest. 69:959-970, 1982). The presence of K1 capsule was monitored by sensitivity to K1-specific bacteriophages. The non-encapsulated strains used represent both random bacteremic isolates and non-encapsulated derivatives of K1-encapsulated strains obtained by selection for resistance to K1-specific phages. We found little or no difference in the sensitivity of K1-encapsulated and non-encapsulated E. coli to killing by neutralized acid extracts of rabbit PMNs. Bacterial killing by these crude fractions can be attributed to the action of BPI because: (i) bacterial killing was blocked by immune (anti-BPI) immunoglobulin but not by preimmune immunoglobulin and (ii) comparison of the dose-response curves of bacterial killing by crude extracts and by purified BPI showed that the bactericidal activity of crude fractions corresponded closely to the BPI content. Human and rabbit BPIs exhibited similar bactericidal potency toward K1-encapsulated E. coli; i.e., <5 mug of either protein killed >90% of 2.5 x 10(7) bacteria. Thus, the potent bactericidal action of BPI toward E. coli is not impeded by K1 capsule, suggesting that the virulence of K1-encapsulated E. coli is a consequence of extracellular survival but not of resistance to intracellular killing.

PubMed Disclaimer

Similar articles

• Killing of gram-negative bacteria by polymorphonuclear leukocytes: role of an O2-independent bactericidal system.
  Weiss J, Victor M, Stendhal O, Elsbach P. Weiss J, et al. J Clin Invest. 1982 Apr;69(4):959-70. doi: 10.1172/jci110535. J Clin Invest. 1982. PMID: 7042758 Free PMC article.
• Impaired innate immunity in the newborn: newborn neutrophils are deficient in bactericidal/permeability-increasing protein.
  Levy O, Martin S, Eichenwald E, Ganz T, Valore E, Carroll SF, Lee K, Goldmann D, Thorne GM. Levy O, et al. Pediatrics. 1999 Dec;104(6):1327-33. doi: 10.1542/peds.104.6.1327. Pediatrics. 1999. PMID: 10585984
• The bactericidal/permeability-increasing protein (BPI), a potent element in host-defense against gram-negative bacteria and lipopolysaccharide.
  Elsbach P, Weiss J. Elsbach P, et al. Immunobiology. 1993 Apr;187(3-5):417-29. doi: 10.1016/S0171-2985(11)80354-2. Immunobiology. 1993. PMID: 8330906 Review.
• The bactericidal/permeability increasing protein of neutrophils is a potent antibacterial and anti-endotoxin agent in vitro and in vivo.
  Elsbach P, Weiss J, Doerfler M, Shu C, Kohn F, Ammons WS, Kung AH, Meszaros KK, Parent JB. Elsbach P, et al. Prog Clin Biol Res. 1994;388:41-51. Prog Clin Biol Res. 1994. PMID: 7831373 Review.

Cited by

• Phenotypic Heterogeneity in Expression of the K1 Polysaccharide Capsule of Uropathogenic Escherichia coli and Downregulation of the Capsule Genes during Growth in Urine.
  King JE, Aal Owaif HA, Jia J, Roberts IS. King JE, et al. Infect Immun. 2015 Jul;83(7):2605-13. doi: 10.1128/IAI.00188-15. Epub 2015 Apr 13. Infect Immun. 2015. PMID: 25870229 Free PMC article.
• Environmental modulation of lipopolysaccharide chain length alters the sensitivity of Escherichia coli to the neutrophil bactericidal/permeability-increasing protein.
  Weiss J, Hutzler M, Kao L. Weiss J, et al. Infect Immun. 1986 Feb;51(2):594-9. doi: 10.1128/iai.51.2.594-599.1986. Infect Immun. 1986. PMID: 3510983 Free PMC article.
• Capsule polysaccharide mediates bacterial resistance to antimicrobial peptides.
  Campos MA, Vargas MA, Regueiro V, Llompart CM, Albertí S, Bengoechea JA. Campos MA, et al. Infect Immun. 2004 Dec;72(12):7107-14. doi: 10.1128/IAI.72.12.7107-7114.2004. Infect Immun. 2004. PMID: 15557634 Free PMC article.
• Shigella flexneri is trapped in polymorphonuclear leukocyte vacuoles and efficiently killed.
  Mandic-Mulec I, Weiss J, Zychlinsky A. Mandic-Mulec I, et al. Infect Immun. 1997 Jan;65(1):110-5. doi: 10.1128/iai.65.1.110-115.1997. Infect Immun. 1997. PMID: 8975899 Free PMC article.
• Endotoxin, capsule, and bacterial attachment contribute to Neisseria meningitidis resistance to the human antimicrobial peptide LL-37.
  Jones A, Geörg M, Maudsdotter L, Jonsson AB. Jones A, et al. J Bacteriol. 2009 Jun;191(12):3861-8. doi: 10.1128/JB.01313-08. Epub 2009 Apr 17. J Bacteriol. 2009. PMID: 19376861 Free PMC article.

References

1. 1. Immunochemistry. 1965 Sep;2(3):235-54 - PubMed
2. 1. J Gen Microbiol. 1972 May;70(3):527-54 - PubMed
3. 1. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(4):481-8 - PubMed
4. 1. J Infect Dis. 1973 Jun;127(6):670-7 - PubMed
5. 1. N Engl J Med. 1974 May 30;290(22):1216-20 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database