Mutants of Escherichia coli lacking ribosomal protein L11 - PubMed (original) (raw)
. 1980 Nov 10;255(21):10517-22.
- PMID: 6776118
Free article
Mutants of Escherichia coli lacking ribosomal protein L11
G Stöffler et al. J Biol Chem. 1980.
Free article
Abstract
Three mutants with ribosomes apparently lacking Protein L11, AM68, AM76, and AM77, were investigated using a variety of immunological techniques to determine whether L11 was indeed lacking. Ouchterlony double diffusion, modified immunoelectrophoresis, and dimer formation on sucrose gradients all gave results indicating Protein L11 was missing from the ribosome in these mutants. Electron micrographs of ribosomes of the mutants were indistinguishable from those of wild type. Ribosomes of AM68, AM76, and AM77, did not bind the antibiotic thiostrepton, but binding was recovered upon reconstitution with wild type Protein L11.