Microinjection of a rabbit beta-globin gene into zygotes and its subsequent expression in adult mice and their offspring - PubMed (original) (raw)

Microinjection of a rabbit beta-globin gene into zygotes and its subsequent expression in adult mice and their offspring

T E Wagner et al. Proc Natl Acad Sci U S A. 1981 Oct.

Abstract

We have transferred a gene coding for rabbit beta-globin into the male pronucleus of mouse zygotes by direct microinjection. Some of these zygotes developed into mature mice which contained this gene and appeared to be producing a rabbit globin. Evidence for the presence of the gene in these animals was provided by Southern blot hybridization analysis. Evidence for the expression of the rabbit gene in these transformed mice and their offspring was provided by hemoglobin isoelectric focusing analysis and specific serological reactivity between mouse anti-rabbit hemoglobin antiserum and a hemolysate from the mice that developed from the microinjected zygotes. The use of this zygote transformation may allow the introduction and expression of a broad range of genetic elements in mammals.

PubMed Disclaimer

Similar articles

• Gene transfer into the mouse germ-line.
  Costantini F, Lacy E. Costantini F, et al. J Cell Physiol Suppl. 1982;1:219-26. doi: 10.1002/jcp.1041130430. J Cell Physiol Suppl. 1982. PMID: 6950943
• Replication and expression of thymidine kinase and human globin genes microinjected into mouse fibroblasts.
  Anderson WF, Killos L, Sanders-Haigh L, Kretschmer PJ, Diacumakos EG. Anderson WF, et al. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5399-403. doi: 10.1073/pnas.77.9.5399. Proc Natl Acad Sci U S A. 1980. PMID: 6254080 Free PMC article.
• Abnormal expression of chromosomal rabbit beta-globin gene in Saccharomyces cerevisiae.
  Beggs JD, van den Berg J, van Ooyen A, Weissmann C. Beggs JD, et al. Nature. 1980 Feb 28;283(5750):835-40. doi: 10.1038/283835a0. Nature. 1980. PMID: 6987524
• Rabbit beta-globin mRNA production in mouse L cells transformed with cloned rabbit beta-globin chromosomal DNA.
  Mantei N, Boll W, Weissmann C. Mantei N, et al. Nature. 1979 Sep 6;281(5726):40-6. doi: 10.1038/281040a0. Nature. 1979. PMID: 551266
• Recovery of recombinant bacterial plasmids from E. coli transformed with DNA from microinjected mouse cells.
  Kretschmer PJ, Bowman AH, Huberman MH, Sanders-Haigh L, Killos L, Anderson WF. Kretschmer PJ, et al. Nucleic Acids Res. 1981 Nov 25;9(22):6199-217. doi: 10.1093/nar/9.22.6199. Nucleic Acids Res. 1981. PMID: 6273826 Free PMC article.

Cited by

• Inhibition of Moloney murine leukemia virus-induced leukemia in transgenic mice expressing antisense RNA complementary to the retroviral packaging sequences.
  Han L, Yun JS, Wagner TE. Han L, et al. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4313-7. doi: 10.1073/pnas.88.10.4313. Proc Natl Acad Sci U S A. 1991. PMID: 2034674 Free PMC article.
• Germ-line transformation of mice.
  Palmiter RD, Brinster RL. Palmiter RD, et al. Annu Rev Genet. 1986;20:465-99. doi: 10.1146/annurev.ge.20.120186.002341. Annu Rev Genet. 1986. PMID: 3545063 Free PMC article. Review. No abstract available.
• GI GEMs: genetically engineered mouse models of gastrointestinal disease.
  Doetschman T. Doetschman T. Gastroenterology. 2011 Feb;140(2):380-385.e2. doi: 10.1053/j.gastro.2010.12.013. Epub 2010 Dec 15. Gastroenterology. 2011. PMID: 21167162 Free PMC article. Review. No abstract available.
• Tissue-specific and hormonally regulated expression of a rat alpha 2u globulin gene in transgenic mice.
  Soares Vda C, Gubits RM, Feigelson P, Costantini F. Soares Vda C, et al. Mol Cell Biol. 1987 Oct;7(10):3749-58. doi: 10.1128/mcb.7.10.3749-3758.1987. Mol Cell Biol. 1987. PMID: 2446121 Free PMC article.
• Direct transfection of viral and plasmid DNA into the liver or spleen of mice.
  Dubensky TW, Campbell BA, Villarreal LP. Dubensky TW, et al. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7529-33. doi: 10.1073/pnas.81.23.7529. Proc Natl Acad Sci U S A. 1984. PMID: 6095303 Free PMC article.

References

1. 1. Cell. 1978 Jul;14(3):725-31 - PubMed
2. 1. J Exp Zool. 1978 Mar;203(3):371-80 - PubMed
3. 1. Arch Androl. 1978;1(1):31-41 - PubMed
4. 1. Proc Natl Acad Sci U S A. 1979 Feb;76(2):867-71 - PubMed
5. 1. Mol Gen Genet. 1979 Feb 26;170(2):179-85 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database

• Medical

  • MedlinePlus Health Information

• Molecular Biology Databases

  • Mouse Genome Informatics (MGI)