Conserved 5S rRNA complement to tRNA is not required for protein synthesis - PubMed (original) (raw)

Conserved 5S rRNA complement to tRNA is not required for protein synthesis

B Pace et al. Proc Natl Acad Sci U S A. 1982 Jan.

Abstract

The notion that tRNA and 5S rRNA interact through evolutionarily conserved complementary sequences has been tested by nucleolytic modification of the 5S rRNA, using the modified rRNA to reconstitute the large ribosomal subunit, and assaying for poly(uridylic acid)-directed polyphenylalanine synthesis. The 5S rRNA sequence C-G-A-A (residues 43-46) and several residues surrounding it are not essential for protein synthesis.

PubMed Disclaimer

Similar articles

• The conserved 5 S rRNA complement to tRNA is not required for translation of natural mRNA.
  Zagorska L, Van Duin J, Noller HF, Pace B, Johnson KD, Pace NR. Zagorska L, et al. J Biol Chem. 1984 Mar 10;259(5):2798-802. J Biol Chem. 1984. PMID: 6365917
• Oligonucleotide directed mutagenesis of Escherichia coli 5S ribosomal RNA: construction of mutant and structural analysis.
  Göringer HU, Wagner R, Jacob WF, Dahlberg AE, Zwieb C. Göringer HU, et al. Nucleic Acids Res. 1984 Sep 25;12(18):6935-50. doi: 10.1093/nar/12.18.6935. Nucleic Acids Res. 1984. PMID: 6091046 Free PMC article.
• Structural analysis and in vitro processing to p5 rRNA of a 9S RNA molecule isolated from an rne mutant of E. coli.
  Ghora BK, Apirion D. Ghora BK, et al. Cell. 1978 Nov;15(3):1055-66. doi: 10.1016/0092-8674(78)90289-1. Cell. 1978. PMID: 365352
• tRNA-rRNA sequence matches from inter- and intraspecies comparisons suggest common origins for the two RNAs.
  Bloch DP, McArthur B, Guimarães RC, Smith J, Staves MP. Bloch DP, et al. Braz J Med Biol Res. 1989;22(8):931-44. Braz J Med Biol Res. 1989. PMID: 2699261 Review.

Cited by

• The conserved 900 stem/loop region in Escherichia coli 16S ribosomal RNA is not required for protein synthesis.
  Gravel M, Leclerc D, Melançon P, Brakier-Gingras L. Gravel M, et al. Nucleic Acids Res. 1989 Apr 11;17(7):2723-32. doi: 10.1093/nar/17.7.2723. Nucleic Acids Res. 1989. PMID: 2654884 Free PMC article.
• Phylogenetic analysis of the spirochetes.
  Paster BJ, Dewhirst FE, Weisburg WG, Tordoff LA, Fraser GJ, Hespell RB, Stanton TB, Zablen L, Mandelco L, Woese CR. Paster BJ, et al. J Bacteriol. 1991 Oct;173(19):6101-9. doi: 10.1128/jb.173.19.6101-6109.1991. J Bacteriol. 1991. PMID: 1917844 Free PMC article.
• Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells.
  Giovannoni SJ, DeLong EF, Olsen GJ, Pace NR. Giovannoni SJ, et al. J Bacteriol. 1988 Feb;170(2):720-6. doi: 10.1128/jb.170.2.720-726.1988. J Bacteriol. 1988. PMID: 2448289 Free PMC article.
• Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology.
  Amann RI, Krumholz L, Stahl DA. Amann RI, et al. J Bacteriol. 1990 Feb;172(2):762-70. doi: 10.1128/jb.172.2.762-770.1990. J Bacteriol. 1990. PMID: 1688842 Free PMC article.
• Phylogeny of Bacteroides, Prevotella, and Porphyromonas spp. and related bacteria.
  Paster BJ, Dewhirst FE, Olsen I, Fraser GJ. Paster BJ, et al. J Bacteriol. 1994 Feb;176(3):725-32. doi: 10.1128/jb.176.3.725-732.1994. J Bacteriol. 1994. PMID: 8300528 Free PMC article.

References

1. 1. Science. 1967 Dec 29;158(3809):1695-9 - PubMed
2. 1. J Biol Chem. 1969 Nov 25;244(22):6241-53 - PubMed
3. 1. J Mol Biol. 1968 Jun 28;34(3):379-412 - PubMed
4. 1. Biochem Biophys Res Commun. 1973 Oct 1;54(3):942-8 - PubMed
5. 1. Anal Biochem. 1974 May;59(1):162-4 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Molecular Biology Databases

  • BioCyc