Covalently closed circles of adenovirus 5 DNA - PubMed (original) (raw)
. 1983 Jan 13;301(5896):172-4.
doi: 10.1038/301172a0.
- PMID: 6823294
- DOI: 10.1038/301172a0
Covalently closed circles of adenovirus 5 DNA
M Ruben et al. Nature. 1983.
Abstract
The genome of adenoviruses is a double-stranded linear DNA molecule with inverted terminal repeats about 100 base pairs (bp) in length and a terminal protein covalently linked to the 5' nucleotide of each strand. Both of these features permit the formation of DNA circles, the inverted repeats allowing the circularization of single-stranded DNA and the terminal protein the joining of one or more molecules to yield double-stranded circles or concatemers. However, although the existence of covalently closed circles has been postulated, double-stranded viral DNA purified from virions or infected cells by conventional methods (that is, using proteases and phenol or chloroform) has always been obtained in a linear form. Here, we present evidence for the existence in adenovirus 5 (Ad5) infected cells of novel structures resulting from covalent head-to-tail joining of viral DNA molecules and show that these structures are due at least in part to the formation of covalently closed circles.
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