Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei - PubMed (original) (raw)

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

J D Dignam et al. Nucleic Acids Res. 1983.

Free PMC article

Abstract

We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. The extract also directs accurate transcription initiation from other adenovirus promoters and cellular promoters. The extract also directs accurate transcription initiation from class III promoters (tRNA and Ad 2 VA).

PubMed Disclaimer

Similar articles

• Separation and characterization of factors mediating accurate transcription by RNA polymerase II.
  Samuels M, Fire A, Sharp PA. Samuels M, et al. J Biol Chem. 1982 Dec 10;257(23):14419-27. J Biol Chem. 1982. PMID: 7142220
• Multiple factors required for accurate initiation of transcription by purified RNA polymerase II.
  Matsui T, Segall J, Weil PA, Roeder RG. Matsui T, et al. J Biol Chem. 1980 Dec 25;255(24):11992-6. J Biol Chem. 1980. PMID: 7440580
• Transcription of viral genes by RNA polymerase II in nuclei isolated from adenovirus 2 transformed cells.
  Bitter GA, Roeder RG. Bitter GA, et al. Biochemistry. 1978 May 30;17(11):2198-205. doi: 10.1021/bi00604a028. Biochemistry. 1978. PMID: 667018 No abstract available.
• Cyclic AMP-mediated protein kinase activation and its regulatory effect on mammalian RNA polymerase.
  Jungmann RA, Kranias EG. Jungmann RA, et al. Adv Biochem Psychopharmacol. 1976;15:413-28. Adv Biochem Psychopharmacol. 1976. PMID: 192057 Review. No abstract available.
• Function, structure, and regulation of eukaryotic nuclear RNA polymerases.
  Roeder RG, Schwartz LB, Sklar VE. Roeder RG, et al. Symp Soc Dev Biol. 1976;(34):29-52. Symp Soc Dev Biol. 1976. PMID: 798342 Review. No abstract available.

Cited by

• Transcriptional properties of mammalian elongin A and its role in stress response.
  Kawauchi J, Inoue M, Fukuda M, Uchida Y, Yasukawa T, Conaway RC, Conaway JW, Aso T, Kitajima S. Kawauchi J, et al. J Biol Chem. 2013 Aug 23;288(34):24302-15. doi: 10.1074/jbc.M113.496703. Epub 2013 Jul 3. J Biol Chem. 2013. PMID: 23828199 Free PMC article.
• An exon splice enhancer primes IGF2:IGF2R binding site structure and function evolution.
  Williams C, Hoppe HJ, Rezgui D, Strickland M, Forbes BE, Grutzner F, Frago S, Ellis RZ, Wattana-Amorn P, Prince SN, Zaccheo OJ, Nolan CM, Mungall AJ, Jones EY, Crump MP, Hassan AB. Williams C, et al. Science. 2012 Nov 30;338(6111):1209-13. doi: 10.1126/science.1228633. Science. 2012. PMID: 23197533 Free PMC article.
• Dental pulp stem cells differentiation reveals new insights in Oct4A dynamics.
  Ferro F, Spelat R, D'Aurizio F, Puppato E, Pandolfi M, Beltrami AP, Cesselli D, Falini G, Beltrami CA, Curcio F. Ferro F, et al. PLoS One. 2012;7(7):e41774. doi: 10.1371/journal.pone.0041774. Epub 2012 Jul 23. PLoS One. 2012. PMID: 22844522 Free PMC article.
• Regulation of zinc-responsive Slc39a5 (Zip5) translation is mediated by conserved elements in the 3'-untranslated region.
  Weaver BP, Andrews GK. Weaver BP, et al. Biometals. 2012 Apr;25(2):319-35. doi: 10.1007/s10534-011-9508-4. Epub 2011 Nov 24. Biometals. 2012. PMID: 22113231 Free PMC article.
• Contrasting expression patterns of coding and noncoding parts of the human genome upon oxidative stress.
  Giannakakis A, Zhang J, Jenjaroenpun P, Nama S, Zainolabidin N, Aau MY, Yarmishyn AA, Vaz C, Ivshina AV, Grinchuk OV, Voorhoeve M, Vardy LA, Sampath P, Kuznetsov VA, Kurochkin IV, Guccione E. Giannakakis A, et al. Sci Rep. 2015 May 29;5:9737. doi: 10.1038/srep09737. Sci Rep. 2015. PMID: 26024509 Free PMC article.

References

1. 1. J Biol Chem. 1973 Jun 10;248(11):3777-88 - PubMed
2. 1. Proc Natl Acad Sci U S A. 1974 May;71(5):1803-7 - PubMed
3. 1. Biochemistry. 1977 May 31;16(11):2334-43 - PubMed
4. 1. J Biol Chem. 1979 Jul 10;254(13):6163-73 - PubMed
5. 1. Cell. 1979 Oct;18(2):469-84 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations Database