Evidence for granulocyte-mediated macrophage activation after C. parvum immunization - PubMed (original) (raw)
Evidence for granulocyte-mediated macrophage activation after C. parvum immunization
S K Chapes et al. Cell Immunol. 1983.
Abstract
It has been previously demonstrated that at the peak of the peritoneal response to Corynebacterium parvum (Day 4), cytolytic macrophages can be characterized by the presence of intracellular bacteria. In the present study, the role of neutrophils in the activation of peritoneal macrophages by C. parvum was investigated. Inflammatory neutrophils isolated 5 hr after ip administration of C. parvum were transferred to normal, syngeneic mice and the peritoneal macrophages of recipients harvested 4 days later were tested for cytoxicity against HeLa cells. Neutrophils isolated from mice 5 hr after C. parvum immunization were effective in inducing cytolytic macrophages. Less than 100-fold as much bacteria was needed to induce comparable levels of cytotoxic activity when introduced inside granulocytes. Neutrophils obtained from mice 48 hr after C. parvum injection or mononuclear cells were not good macrophage activators. Viable neutrophils were not required as freeze-thawed cells were able to activate macrophages in recipient mice. The intracellular distribution of C. parvum changed dramatically with time. Initially almost all bacteria were found within neutrophils. By 24 hr, many macrophages contained either bacteria or granulocytes which had ingested C. parvum. Pyridine extracts of C. parvum, which do not activate peritoneal macrophages when injected directly into mice, did not induce neutrophils capable of activating macrophages. The residue of pyridine-extracted C. parvum did induce neutrophils that could activate macrophages when transferred. The results suggest that processing of the bacteria by inflammatory granulocytes may be an obligatory step in macrophage activation by this agent. The peak response occurred earlier than T-cell immunity is usually observed and it is suggested that direct activation of macrophages via ingestion of neutrophils may represent the earliest stage of macrophage activation by C. parvum.
Similar articles
Cited by
- Evaluation of macrophage plasticity in brown and white adipose tissue.
Ortega MT, Xie L, Mora S, Chapes SK. Ortega MT, et al. Cell Immunol. 2011;271(1):124-33. doi: 10.1016/j.cellimm.2011.06.012. Epub 2011 Jun 23. Cell Immunol. 2011. PMID: 21757190 Free PMC article. - Effects of supernatants and lysates of polymorphonuclear leucocytes: macrophage stimulatory factors.
Bird J, Sheng YJ, Giroud JP. Bird J, et al. Br J Exp Pathol. 1984 Apr;65(2):243-50. Br J Exp Pathol. 1984. PMID: 6370291 Free PMC article. - Antitumor effect induced by a hot water extract of Chlorella vulgaris (CE): resistance to Meth-A tumor growth mediated by CE-induced polymorphonuclear leukocytes.
Konishi F, Tanaka K, Himeno K, Taniguchi K, Nomoto K. Konishi F, et al. Cancer Immunol Immunother. 1985;19(2):73-8. doi: 10.1007/BF00199712. Cancer Immunol Immunother. 1985. PMID: 3845850 Free PMC article. - Antitumor and immunologic effects of a pyridine-extracted fraction of Propionibacterium acnes.
Lichtenstein A, Berek J. Lichtenstein A, et al. Cancer Immunol Immunother. 1986;22(1):24-30. doi: 10.1007/BF00205712. Cancer Immunol Immunother. 1986. PMID: 3708631 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical