Establishment and characterization of human pancreatic adenocarcinoma cell line SW-1990 in tissue culture and the nude mouse - PubMed (original) (raw)

. 1983 Sep;43(9):4393-401.

• PMID: 6871872

Establishment and characterization of human pancreatic adenocarcinoma cell line SW-1990 in tissue culture and the nude mouse

A P Kyriazis et al. Cancer Res. 1983 Sep.

Abstract

A new tumor line derived from a human pancreatic ductal adenocarcinoma of a 56-year-old Caucasian male was established in tissue culture and the nude mouse. In tissue culture, the neoplastic cells grew as large, epithelial-like, mucin-producing cells. Injection s.c. of 1 X 10(6) cultured neoplastic cells into nude mice resulted in tumor formation histologically closely resembling the original neoplasm. Ultrastructurally, the neoplastic cells showed characteristics of ductal epithelium. The allozyme phenotypic profile of the line was established in 14 genetically determined loci, and chromosome studies showed a near-tetraploid pattern. Production of macromolecules such as lactate dehydrogenase and carcinoembryonic antigen were present in measurable amounts in culture media, tumor cell extracts, nude mouse-grown tumors, and the serum of tumor-bearing mice in amounts relative to tumor size. Pancreatic enzymes were not detected. These characteristics make tumor line SW-1990 a valuable tool in studying various aspects of the biology of human pancreatic cancer.

PubMed Disclaimer

Similar articles

• [Establishment and characterization of human pancreatic adenocarcinoma cell line SOJ producing carcinoembryonic antigen and carbohydrate antigen 19-9].
  Fujii Y, Sekiguchi M, Shiroko Y, Shimizu H, Sugawara I, Hasumi K, Eriguchi M, Ikeuchi T, Uchida H. Fujii Y, et al. Hum Cell. 1990 Mar;3(1):31-6. Hum Cell. 1990. PMID: 2083222 Japanese.
• Tissue culture and transplantation of breast cancer.
  Ozzello L. Ozzello L. Prog Clin Biol Res. 1977;12:55-70. Prog Clin Biol Res. 1977. PMID: 335406 Review. No abstract available.
• [Tissue culture methods of hormone-producing tumors].
  Eto S. Eto S. Nihon Rinsho. 1983 Jun;41(6):1314-23. Nihon Rinsho. 1983. PMID: 6312132 Review. Japanese. No abstract available.

Cited by

• Kinetics of carcinoembryonic antigen and carbohydrate antigen 19-9 production in a human pancreatic cancer cell line (SUIT-2).
  Iwamura T, Katsuki T. Iwamura T, et al. Gastroenterol Jpn. 1987 Oct;22(5):640-6. doi: 10.1007/BF02776726. Gastroenterol Jpn. 1987. PMID: 2445616
• Treatment of pancreatic cancer in a nude mouse model using high-intensity focused ultrasound.
  Jiang L, Hu B, Guo Q, Chen L. Jiang L, et al. Exp Ther Med. 2013 Jan;5(1):39-44. doi: 10.3892/etm.2012.744. Epub 2012 Oct 10. Exp Ther Med. 2013. PMID: 23251239 Free PMC article.
• Differential mucin gene expression in human pancreatic and colon cancer cells.
  Yonezawa S, Byrd JC, Dahiya R, Ho JJ, Gum JR, Griffiths B, Swallow DM, Kim YS. Yonezawa S, et al. Biochem J. 1991 Jun 15;276 ( Pt 3)(Pt 3):599-605. doi: 10.1042/bj2760599. Biochem J. 1991. PMID: 2064602 Free PMC article.
• Human endometrium transplanted into nude mice. Histologic effects of various steroid hormones.
  Bergqvist A, Jeppsson S, Kullander S, Ljungberg O. Bergqvist A, et al. Am J Pathol. 1985 May;119(2):336-44. Am J Pathol. 1985. PMID: 3158210 Free PMC article.
• Cyclic-AMP-stimulated synthesis and release of carcinoembryonic antigen by pancreatic cancer cells.
  Sack TL, Gum JR, Kim YS. Sack TL, et al. Int J Pancreatol. 1988 Mar;3(2-3):171-84. doi: 10.1007/BF02798929. Int J Pancreatol. 1988. PMID: 2834472

Publication types

MeSH terms

Substances

LinkOut - more resources

• Medical

  • MedlinePlus Health Information

• Research Materials

  • Cellosaurus - a cell line knowledge resource