Glutamine metabolism in lymphocytes of the rat - PubMed (original) (raw)

Glutamine metabolism in lymphocytes of the rat

M S Ardawi et al. Biochem J. 1983.

Abstract

The metabolism of glutamine in resting and concanavalin-A-stimulated lymphocytes was investigated. In incubated lymphocytes isolated from rat mesenteric lymph nodes, the rates of oxygen and glutamine utilization and that of aspartate production were approximately linear with respect to time for 60 min, and the concentrations of adenine nucleotides plus the ATP/ADP or ATP/AMP concentration ratios remained approximately constant for 90 min. The major end products of glutamine metabolism were glutamate, aspartate and ammonia: the carbon from glutamine may contribute about 30% to respiration. When both glucose and glutamine were presented to the cells, the rates of utilization of both substances increased. Evidence was obtained that the stimulation of glycolysis by glutamine could be due, in part, to an activation of 6-phosphofructokinase. Starvation of the donor animal increased the rate of glutamine utilization. The phosphoenolpyruvate carboxykinase inhibitor mercaptopicolinate decreased the rate of glutamine utilization by 28%; the rates of accumulation of glutamate and ammonia were decreased, whereas those of lactate, aspartate and malate were increased. The mitogen concanavalin A increased the rate of glutamine utilization (by about 51%). The rate of [3H]thymidine incorporation into DNA caused by concanavalin A in cultured lymphocytes was very low in the absence of glutamine; it was increased about 4-fold at 1 microM-glutamine and was maximal at 0.3 mM-glutamine; neither other amino acids nor ammonia could replace glutamine.

PubMed Disclaimer

Cited by

Exercise and the immune response.
Keast D, Cameron K, Morton AR. Keast D, et al. Sports Med. 1988 Apr;5(4):248-67. doi: 10.2165/00007256-198805040-00004. Sports Med. 1988. PMID: 3287548 Review.
Fuel utilization in colonocytes of the rat.
Ardawi MS, Newsholme EA. Ardawi MS, et al. Biochem J. 1985 Nov 1;231(3):713-9. doi: 10.1042/bj2310713. Biochem J. 1985. PMID: 4074334 Free PMC article.
Glutamine depletion potentiates leucocyte-dependent inflammatory events induced by carrageenan or Clostridium difficile toxin A in rats.
Nascimento SB, Sousa RB, Martins MJ, Souza Gomes A, Souza MH, Guerrant RL, Cunha FQ, Ribeiro RA, Brito GA. Nascimento SB, et al. Immunology. 2005 Nov;116(3):328-36. doi: 10.1111/j.1365-2567.2005.02232.x. Immunology. 2005. PMID: 16236122 Free PMC article.
Monitoring dynamic changes in lymph metabolome of fasting and fed rats by electrospray ionization-ion mobility mass spectrometry (ESI-IMMS).
Kaplan K, Dwivedi P, Davidson S, Yang Q, Tso P, Siems W, Hill HH Jr. Kaplan K, et al. Anal Chem. 2009 Oct 1;81(19):7944-53. doi: 10.1021/ac901030k. Anal Chem. 2009. PMID: 19788315 Free PMC article.
Targeting cancer metabolism: a therapeutic window opens.
Vander Heiden MG. Vander Heiden MG. Nat Rev Drug Discov. 2011 Aug 31;10(9):671-84. doi: 10.1038/nrd3504. Nat Rev Drug Discov. 2011. PMID: 21878982 Review.

References

1. Exp Cell Res. 1973 Mar 15;77(1):127-35 - PubMed
1. J Biol Chem. 1974 Aug 25;249(16):5070-9 - PubMed
1. J Biol Chem. 1976 Jan 10;251(1):37-44 - PubMed
1. Arch Biochem Biophys. 1975 Dec;171(2):459-65 - PubMed
1. Biochem J. 1975 Jun;148(3):583-94 - PubMed

Glutamine metabolism in lymphocytes of the rat - PubMed (original) (raw)