Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line - PubMed (original) (raw)

Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line

P Masiakowski et al. Nucleic Acids Res. 1982.

Abstract

cDNA clones corresponding to a mRNA whose level is rapidly increased by addition of oestradiol to the culture medium have been isolated by differential screening of a cDNA library from the breast cancer cell line MCF-7, which contains oestrogen receptors. Such clones will be useful in studies of the DNA sequences required for hormonal induction and to determine whether expression of the corresponding gene is in any way related to the cancerous state. We have also obtained a cDNA clone for a messenger whose level is apparently decreased by steroid hormones.

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References

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